Tosoh Bioscience

Precisely characterize polymers by using advanced detection and analytical techniques.

Learn the importance of light scattering detection to determine the true molecular weight distribution of a poly(glutamic acid)-based copolymer for drug-delivery.

Airing 1: Thursday November 10, 2022 at 9am EST Airing 2: Thursday November 10, 2022 at 2pm EST Polymer characterization is key to understanding their properties and ensuring the quality of final products containing polymers. Discover why light scattering and viscometry in size-exclusion chromatography become the new gold standard in polymer analysis.

This application note explains how SEC coupled with MALS can be used for the determination of structural changes in polysaccharides.

Thursday, October, 20, 2022 at 9 am EDT | 3 pm CEST Do you rely on size-exclusion chromatography for the analysis of proteins? Then tune in to learn the basics of SEC and method optimization tips from our chromatography expert.

Biotherapeutics such as monoclonal antibody-derived molecules as well as their impurity profiles are becoming more complex. That challenges the routinely applied analysis of high- and low-molecular weight impurities by size exclusion chromatography and UV-absorbance. Possible limitations of SEC-UV can be overcome by adding advanced detection technologies such as multiangle light scattering (MALS) and mass spectrometry (MS). This podcast will explain the different detection methods and the results they deliver. Special emphasis will be placed on the higher requirements for size exclusion columns and methods preceding advanced detection methods.

Monoclonal antibodies (mAbs) are effective therapeutics for cancers, auto-immune diseases, viral infections, and other diseases. Recent developments in antibody therapeutics aim to add more specific binding regions (bi- and multi-specificity) to increase their effectiveness and/or to downsize the molecule to the specific binding regions (for example, scFv or Fab fragment) to achieve better penetration of the tissue. As the molecule gets more complex, the possible high and low molecular weight (H/LMW) impurities become more complex, too. In order to accurately analyze the various species, more advanced detection than ultraviolet (UV) is required to characterize a mAb sample.

Thursday, April 7, 2022 at 9am EST | 6am PST | 2pm GMT | 3pm CET Thursday, April 7, 2022 at 2pm EST | 11am PST | 7pm GMT | 8pm CET This webinar will present an analytical method to determine several key AAV quality attributes (capsid titer, absolute molecular weight, empty/full ratio, size, and aggregate content) using size-exclusion chromatography (SEC) coupled to multi-angle light scattering (MALS).

To monitor FcR receptor binding, a recombinant FcγRIIIa-ligand has been commercialized into TSKgel FcR-IIIA high-performance affinity chromatography (AFC) columns that separate antibodies based on affinity differences to FcγRIIIa.

Polymers for life-science applications have different attributes from traditional applications such as automotive and packaging. These attributes have their own set of challenges in the characterization requirements of such complex molecules. Multi-angle light scattering (MALS) technology provides a more insightful tool.

Wednesday, November 3, 2021 at 11am EDT | 10am CDT | 3pm GMT | 4pm CET Learn a new and effective method to assess drug potency of mAbs and ADCs. FcR-IIIA affinity chromatography is the latest technique that can profile mAbs and their underlying glycan pattern.

Biomolecules such as proteins, antibodies or viral vectors are becoming increasingly important therapeutics, as evidenced by clinical trials and ultimately drug approvals. But they are also significantly different from small molecules, which affects their HPLC analysis. We explain the differences and what that means for the HPLC conditions and modes used to analyze biomolecules.

This application note demonstrates the scalability from analytical to semi-preparative mode while maintaining similar resolution between the two different capacity columns.

A new approach to assess glycan profiles during cell line screening.

This two-step workflow allows for the rapid screening of N-linked glycans for upstream and downstream mAb production.