Advanced detection (MALS, MS) for size exclusion analysis of proteins - what it offers and what it demands
Biotherapeutics such as monoclonal antibody-derived molecules as well as their impurity profiles are becoming more complex. That challenges the routinely applied analysis of high- and low-molecular weight impurities by size exclusion chromatography and UV-absorbance. Possible limitations of SEC-UV can be overcome by adding advanced detection technologies such as multiangle light scattering (MALS) and mass spectrometry (MS). This podcast will explain the different detection methods and the results they deliver. Special emphasis will be placed on the higher requirements for size exclusion columns and methods preceding advanced detection methods.
Extraction of 40 PFAS Compounds from Soil and Tissue
December 6th 2024PFAS have been shown to cause health issues in humans, which means monitoring environmental solid samples, such as soil and tissue, is critical. This application note details the extraction of 40 spiked PFAS compounds from soil and tissue following EPA Method 1633. The automated extraction was less than 10 minutes per sample and yielded acceptable recoveries and RSDs without carryover in the system. The EDGE PFAS is an ideal option for laboratories that want to automate their PFAS extractions of solid samples.
