Tosoh Bioscience

This application note demonstrates that FcγRIIIA affinity chromatography allows fast assessment of biologic activity and glycoform pattern of antibodies.

The need for enhanced product quality of biomolecules has led to a higher-level of characterization of different critical quality attributes. Size exclusion chromatography (SEC) has been widely used as a technique to provide size and purity. SEC coupled with multi-angle light scattering (MALS) can determine molecular weight (MW) and size (or radius of gyration, Rg) of complex samples. Dr. Wei Lu, Ph.D. and Philip Hoang from Tosoh Bioscience discuss how SEC-MALS as an analytical method can be optimized and applied to a wide range of applications including oligonucleotides, monoclonal antibodies, and virus-like particles.

*Tuesday, June 1, 2021 at 12:30pm BST| 1:30pm CEST| 7:30am EDT & Wednesday, June 2, 2021 at 12:30pm BST| 1:30pm CEST| 7:30am EDT* John Knox played a pivotal role in the evolution and understanding of both gas and liquid chromatography and is perhaps best known for the development of the Knox Equation, which describes the different contributions to overall band broadening of a solute. *On Demand June 2, 2021*

***Thursday and Friday May 13-14 | May 20-21, 2021*** In the relaxed and unconventional talks in this symposium, highly experienced speakers from industry and academia will impart key learnings from their long experience with chromatography—from heroic failures that have taught them valuable lessons, to moments of inspirational serendipity, and everything in between. CHROMTalks will deliver lifetimes of learning in condensed form, covering topics across implementation, troubleshooting, and method development of analytical techniques. Time spent at CHROMTalks will pay back as soon as you enter your laboratory! ***Available On Demand Until May 21, 2022***

*** Wednesday, May 5, 2021 at 11am EDT | 8am PDT | 4pm BST | 5pm CEST*** A guide to enable users to acquire high quality intact protein mass spectra by inline SEC-ESI-MS will be presented. Gain knowledge on the use and optimization of volatile mass spectrometry-friendly buffers in U/HPLC-MS methods for the analysis of intact mAbs. *On demand available after final airing until May 5, 2022*

Combining high-resolution size exclusion chromatography columns with highly sensitive MALS detection characterizes oligonucleotides and structurally-related impurities.

***Live: Tuesday, March 16, 2021 at 11am EDT | 8am PDT | 3pm GMT | 4pm CET*** In this webcast we will provide our top 10 tips for selecting HPLC columns. We will consider everything from analyte-stationary phase interactions, to solid support materials, and how column dimensions can impact separations; and everything in between! *** On demand available after final airing until Mar. 31, 2021.***

AEX using a TSKgel® DNA-NPR column offers a rapid method for discriminating between covalently closed circular, open circular, and linear forms of plasmid DNA.

***Live Dates:Day 1, Wednesday, November 18, 2020 9am–11:30am EST | 2pm – 3:30pm GMT | 3pm-4:30pm CET Day 2, Thursday, November 19, 2020 9am–11:30am EST | 2pm – 3:30pm GMT | 3pm-4:30pm CET ***Biopharmaceutical analysis is a fast-paced and constantly evolving field, and chromatographers play a pivotal role. This two-day virtual event, focusing on cutting-edge trends, has been organized by The Chromatographic Society (“ChromSoc”) in collaboration with LCGC and features talks from top analytical chemists working in biopharmaceutical analysis.***On demand available after final airing until Nov. 19, 2021***

Elution profiles based on mAb glycan structures produced by the TSKgel® FcR-IIIA-NPR™ affinity chromatography column correlate with results from HILIC glycan profiling.  

***Live: Wednesday, October 28, 2020, 11am EDT| 8am PDT| 3pm GMT| 4pm CET*** Discover a new and unique tool to assess the variances in mAb glycoforms and their differences in potency and efficacy using an affinity Fc-Receptor based chromatographic methodology. Data obtained can be correlated to ADCC activity. *** On demand available after final airing until Oct. 28, 2021.***

***Live: Two Day Event! Day One: Thursday, Oct. 22, 2020 10am EDT | 12:30pm EDT | 2pm EDT & Day Two: Friday, Oct. 23, 2020 10am EDT | 2pm EDT *** In this two-day online event, speakers will discuss theory and applications of gas chromatography (GC) and high performance liquid chromatography (HPLC). We have a morning and afternoon session each day, plus a lunchtime tutorial session on 2D-GC on Day 1. There will be live Q&A for the morning sessions and the tutorial. Attendance is free..*** On demand available after final airing until Oct. 23, 2021.***

How UHP‑SEC‑MALS analysis using a UHPLC compatible multi-angle light scattering detector can be applied to determine absolute molecular weight of recombinant proteins.

Online detection of absolute molecular weight of two recombinant protein samples using a 2 μm TSKgel® SEC column directly connected to the LenS3™MALS detector is shown.

Having a good troubleshooting strategy which can be applied to all problems is essential, therefore, using examples of real-life chromatographic problems we will provide you with the knowledge you need to become the expert troubleshooter in your lab. Live: Wednesday, March 18, 2020 at 11am EDT | 8am PDT | 3pm GMT | 4pm CET On demand available after final airing until Mar. 31, 2020 Register free

The molecular similarity between Humira® and Yervoy® biosimilars and the corresponding innovator reference products is examined using analytical SEC, RPC and HIC.

The oligonucleotide therapeutics field has seen remarkable progress over the last few years and oligonucleotides are increasingly recognized as potential therapeutic agents for a variety of diseases. Here we describe the ability of ultra-high pressure size exclusion chromatography (SEC) to distinguish N and N-1 oligonucleotide species.

Multidimensional LC/MS is a powerful tool to gain info in the laboratory. Analyzing ADCs with multidimensional LC/MS provides fast data for CQAs.