The Application Notebook-02-11-2011

February 11, 2011

The isolation of α-Methylstyrene from the crude reaction mixture seems easy. However the reaction mixture contains some dimethyl sulfoxide and the oily wetting agent from the sodiumhydride.

February 11, 2011

Phospholipids are important compounds for the formation of bilayer membranes in biological systems.

February 11, 2011

The demand for faster throughput in LC–MS drug discovery labs has increased throughout the years.

February 11, 2011

BioPharma Compass is a fully automated solution for the rapid characterization of biopharmaceutical products such as proteins, peptides, RNA, and DNA.

February 11, 2011

Application of core-shell technology to the USP assay for ibuprofen to reduce analysis time and increase sample throughput and productivity, while improving the sensitivity of the assay for the determination of ibuprofen related compound C, a known degradation product with adverse health effects.

February 11, 2011

A simple and efficient supported liquid extraction (SLE) procedure for the extraction of THC and metabolites from human plasma at low levels has been developed.

February 11, 2011

The method described employs a triple quadrupole mass spectrometer equipped with hyperbolic quadrupole rods for increased selectivity to prerform a trace level screening method for PCDD/Fs and PCBs and follows the well-established United States Environmental Protection Agency (US EPA) Method 1613A.

February 11, 2011

Tablet formulations of most major pharmaceutical drug products contain significant amounts of inactive ingredients (excipients) in their formulations.

February 11, 2011

The QuEChERS method of food sample preparation was used in conjunction with comprehensive two-dimensional gas chromatography time-of-flight mass spectrometry (GCxGC–TOFMS) to successfully identify PAH contaminants spiked in liquid commercial infant formula.

February 11, 2011

AGPC column and organic solvents are used to selectively pass large macro molecules such as lipids, resins, waxes, and other compounds from sample extracts while retaining the smaller target analyte molecules in the pores of the gel.

February 11, 2011

Recognition of DNAs and RNAs wide range of biological actions has driven modern biopharmaceutical research into the therapeutic value of oligonucleotides.

February 11, 2011

This rapid LC–MS method shows sensitive and selective separation of five ethanolamine analytes in wastewater.

February 11, 2011

The US Pharmacopeia recently released USP as the current monograph for determining residual solvents in pharmaceutical products by static headspace.

February 11, 2011

An approach was developed to align release and end-expiry specifications for molecular size for the quality control of Merck's polyvalent pneumococcal polysaccharide vaccine (PNEUMOVAX 23).

February 11, 2011

Biodiesel fuels are part of a rapidly growing alternative fuel industry to replace gasoline with renewable, more efficient, and lower emission fuels (1).

February 11, 2011

Flexible synthetic fused silica capillary is routinely supplied to the market in long continuous lengths on low cost Styrofoam or plastic spools.

February 11, 2011

An analytical method for the rapid determination of 25-OH vitamin D2 and 25-OH vitamin D3 in serum, which will speed diagnosis of potential Vitamin D deficiencies, is described.

February 11, 2011

Organochlorine pesticides are man-made organic chemicals with a history of wide spread use in the United States, as well as globally.

February 11, 2011

Aminopolycarboxylate chelating agents form stable complexes with transition metals, thereby increasing metal solubility, preventing metal-catalyzed reactions, and removing heavy metal contamination in soil remediation.

February 11, 2011

Allantoin is a heterocyclic organic compound derived from purine.

February 11, 2011

Gelatin is the denatured form of collagen, the main component of skin, bone, and connective tissues of animals.

February 11, 2011

Clavulanic acid is commonly used in combination with penicillin and cephalosporin antibiotics to overcome bacterial resistance.

February 11, 2011

Sweeteners are low or zero-calorie sugar substitutes that are added in drinks to provide the sweet taste of sucrose or table sugar.

February 11, 2011

Many modern stationary phases suffer from poor chromatographic performance for basic compounds at low pH, especially when using low-ionic strength mobile phases such as formic acid.

February 11, 2011

Natural gas and other gaseous fuels contain varying amounts and types of sulfur compounds which can be corrosive to equipment and can inhibit or destroy gas processing catalysts.

February 11, 2011

This application note demonstrates the extraction of sour cream, cream cheese, coffee creamer, heavy whipping cream, and low fat milk using an ASE 350 Accelerated Solvent Extractor technique developed by Dionex and compares results to the traditional Mojonnier technique.

February 11, 2011

Saccharides are fundamental substances that express various bioactivities and may exist independently or form complexes with proteins or lipids.

February 11, 2011

A modified QuEChERS-type extraction method is used with an additional concentration step to detect 38 anthelmintic (nematicides, flukicides, endectocides) drug residues, consisting of benzimidazoles, avermectins, and flukicides in milk. UHPLC–MS-MS instrumentation detects most analytes at

February 11, 2011

Nicotine is a famously addictive alkaloid found in tobacco. Controlled doses of nicotine compounds are sometimes used to help tobacco users quit smoking.

February 11, 2011

This February 2011 edition of The Application Notebook is the 10th Anniversary of publishing these supplements and further affirms the valuable service they perform in the chromatography community.

February 11, 2011

Perfluorinated compounds (PFC) are considered as emerging contaminants that attract high interest from the international scientific community.

February 11, 2011

Using a bonded polymeric zwitterionic stationary phase, in HILIC mode, polar quaternary herbicides like Diquat can easily be retained, with good peak symmetry.

February 11, 2011

Since the gas flow required for the separation step in gas chromatography is frequently lower that that required to optimize the detection, nitrogen is used as a make-up gas to increase the gas flow for detection.

February 11, 2011

Rapid Resolution High Definition (RRHD) columns, used on UHPLC instruments, provide significant productivity enhancements because they are stable to 1200 bar and can withstand higher flow rates.

February 11, 2011

Pesticide pollution is a subject of global concern, and although many countries have now banned the use of organochlorine pesticides, they linger in the environment and can contaminate water sources.

February 11, 2011

With Americans becoming increasingly health conscious and concerned with their exposure to chemicals, businesses have made movements to create more products that are safer for the consumer and the environment.

February 11, 2011

The separation of closely related compounds is of interest to many disciplines including natural products, flavors and fragrances, and pharmaceuticals.

February 11, 2011

The determination of enantiomeric purity is crucial in new drug development.