LCGC Europe-02-01-2004

The latest newcomer to the collection of GC techniques, GCXGC, is examined in this instalment. Although this technique is capable of delivering more GC information in a shorter time than other methods, its technical complexity has slowed down widespread acceptance. John Hinshaw explains...

Extracting analytes from biological tissues often presents serious challenges for the analytical chemist. In this column, the guest authors provide an overview of human and animal tissue sample preparation, and compare conventional extraction technologies with novel approaches to these challenges.

Peaks that are too broad can mean that analysts are not using their LC columns very efficiently. In this month's column, John Dolan describes how to determine if peaks are broader than they should be and highlights some of the common system-related causes of peak broadening.

In the welcome return of this column, Bob McDowall revisits the evolving topic of 21 CFR 11 compliance, as it applies to chromatographers. He examines the FDA's activities in 2003 and outlines the current status of its regulations over, and guidance for, electronic records issues.

In this article the authors report on a combinatorial natural product discovery methodology that uses a viral vector system to transfer secondary metabolite-related enzymes from C. roseus to tobacco cell cultures. Using high-resolution separation techniques, including HPLC, CE and MS, they describe the analysis of secondary metabolite patterns.