Analysis of Combinatorial Natural Products by HPLC and CE

February 1, 2004
Julia Khandurina

Torrey Mesa Research Institute, 3115 Merryfield Row, San Diego, California 92121.

,
Wenying Xu

Torrey Mesa Research Institute, 3115 Merryfield Row, San Diego, California 92121.

,
Yu-Ming Hou

Torrey Mesa Research Institute, 3115 Merryfield Row, San Diego, California 92121.

,
Paul Budworth

Torrey Mesa Research Institute, 3115 Merryfield Row, San Diego, California 92121.

,
Xun Wang

Torrey Mesa Research Institute, 3115 Merryfield Row, San Diego, California 92121.

,
András Guttman

LCGC Europe

Volume 17, Issue 2
Page Number: 104–111

In this article the authors report on a combinatorial natural product discovery methodology that uses a viral vector system to transfer secondary metabolite-related enzymes from C. roseus to tobacco cell cultures. Using high-resolution separation techniques, including HPLC, CE and MS, they describe the analysis of secondary metabolite patterns.