LCGC Europe-05-01-2018

Comprehensive two-dimensional liquid chromatography (LC×LC) is a powerful technique for separating highly complex samples. However, the proliferation of this technique is hindered by a range of challenges, including the possible impact of the additional separation on the detection sensitivity, concerns that mobile phase incompatibility problems will limit the applicability, and the complexity of the system and associated method development costs. This article addresses these issues and describes how modern modulators and software tools are overcoming the barriers associated with this technique.

The biomimetic gradient retention time measurements on C18, immobilized artificial membrane (IAM), human serum albumin (HSA), and acid-glycoprotein (AGP) stationary phases can be used to characterize compounds partitioning into phospholipids and proteins. The data obtained can then be used in equations to estimate the in vivo plasmaÐtissue distribution of the compounds measured. The plasma protein binding, brain tissue binding, and in vivo drug efficiency can also be calculated using the biomimetic chromatographic data.

With so many choices for fittings to use in making connections in my liquid chromatography (LC) system, how do I know which ones are the right ones for my application?

LCGC Europe
Sample Preparation Perspectives

May 01, 2018

This yearly report on new products introduced at Pittcon (or in the preceding year) covers sample preparation instrumentation, supplies, and accessories.

LCGC Europe

John Hinshaw presents his annual review of new developments in the field of gas chromatography seen at Pittcon and other venues in the past 12 months.

LCGC Europe
Features

May 01, 2018

We explore the careers and achievements of the winners of LCGC’s 11th annual awards: Ronald E. Majors and Zachary S. Breitbach.

The Essentials
LCGC Europe

May 01, 2018

An excerpt from LCGC’s e-learning tutorial on inlet maintenance at CHROMacademy.com

LCGC Europe

The 2018 Sample Prep Summer Course will take place in Chania-Crete, Greece, on 22–24 August 2018.

LCGC Europe

LCGC Europe spoke to Andrew Turner, Principal Chemist in the Food Safety Group at the Centre for Environment, Fisheries, and Aquaculture Science (Cefas) in Weymouth, UK, about developing a simple ultrahigh-pressure liquid chromatography tandem mass spectrometry (UHPLCÐMS/MS) method for the quantitation of microcystins and nodularin in various sample matrices.

Click the title above to open the LCGC Europe May 2018 regular issue, Vol 31, No 5, in an interactive PDF format.