Bob W.J. Pirok | Authors

Articles

Clinical Metabolomics: Expanding the Metabolome Coverage Using Advanced Analytical Techniques

Clinical metabolomics requires analytical methods that provide both high resolution and high throughput. In this review, we assess the promise of a variety of techniques-including HILIC, SFC, multidimensional LC, ion-mobility mass spectrometry, and data-independent acquisition mass spectrometry methods-to meet those needs.

Clinical Metabolomics: Expanding the Metabolome Coverage Using Advanced Analytical Techniques

This review article discusses the novel separation and detection strategies that are considered promising in clinical metabolomics to enhance the metabolome coverage. It includes hydrophilic interaction chromatography (HILIC), supercritical fluid chromatography (SFC), multidimensional LC approaches, as well as ion-mobility mass spectrometry (IM-MS) and data-independent acquisition (DIA) analysis methods.

Practical Approaches to Overcome the Challenges of Comprehensive Two-Dimensional Liquid Chromatography

Comprehensive two-dimensional liquid chromatography (LC×LC) is a powerful technique for separating highly complex samples. However, the proliferation of this technique is hindered by a range of challenges, including the possible impact of the additional separation on the detection sensitivity, concerns that mobile phase incompatibility problems will limit the applicability, and the complexity of the system and associated method development costs. This article addresses these issues and describes how modern modulators and software tools are overcoming the barriers associated with this technique.