LCGC North America-05-01-2017

LCGC North America

Statistics for Analysts Who Hate Statistics, Part VI: Derringer Desirability Functions

May 01, 2017

Peer-Reviewed Articles

35

5

Derringer desirability functions are a great favorite of mine because they are very simple and flexible. They may be applied to a variety of problems: whenever you need to select the “best” (sample, method, operating conditions etc.) from a set. It is also a convenient way to compare apples and oranges, whenever totally unrelated features must be ranked. They were first described by Derringer and Markham1 to select polymeric materials based on varied properties.

Mitigating the Effect of Surfactants in Sample Preparation of a Phospholipid Formulation for Impurities Analysis by Reversed-Phase Liquid Chromatography

May 01, 2017

Peer-Reviewed Articles

35

5

A surfactant based diluent was used in sample preparation for reversed phase HPLC analysis of a drug product and its impurities in a phospholipid formulation. The use of the didodecyl trimethylammonium bromide (DDAB) enabled a consistent, quantitative extraction of the analytes of interest from the lipid matrix in a much more aqueous, weak solvent. Water was added as an anti-solvent to precipitate out the surfactant from the system to eliminate HPLC injection pressure spikes, enabling large volume injections and achieving a consistent, robust method for long term use. Method development and validation steps are described.

Vol 35 No 5 LCGC North America May 2017 Regular Issue PDF

May 01, 2017

Issue PDF

35

5

Click the title above to open the LCGC North America May 2017 regular issue, Vol 35 No 5, in an interactive PDF format.