Blood is perhaps the most widely used sample fluid in bioanalysis. Dried blood spots (DBS) have been used with clinical samples for over 50 years but are recently seeing a resurgence of interest. DBS hold several advantages associated with the use of small sample sizes obtained via finger pricks, reduction biohazard, and more. In the previous installment, we gave an overview of microsampling in bioanalysis. This month, we will dig deeper into bioanalysis using DBS.