LCGC North America-12-01-2014

In this installment of "Sample Prep Perspectives," we cover some of the basic scientific principles behind solid-phase extraction (SPE) to allow the correct mode of extraction to be selected through an understanding of how analytes interact with and are separated by the sorbent.

Make your column last forever? Not quite, but you can help prevent its early demise. 

To obtain sensitive, robust, and reproducible gas chromatography (GC) methods, each stage of the chromatographic process needs to be carefully considered and optimized. It is also important to record and report as much detail within the method specification so that the method can be reproduced between operators, instruments, and laboratories. Table I represents a "blueprint" method specification with all of the information that is necessary to faithfully specify and reproduce a split–splitless GC method.

In this installment, we discuss fluorescence-detection size exclusion chromatography (FSEC) technology and its implications in protein characterization, specifically the development and characterization of biologics.  

Click the title above to open the LCGC North America December 2014 regular issue, Vol 32 No 12, in an interactive PDF format.

This article reports on the physical phenomena that control column efficiency and on experimental protocols designed to accurately measure their contributions to band broadening of analytes during their passage from the injection to the detection device. The results of these protocols are analyzed, allowing for the accurate determination of the complete mass transfer mechanism in different separation modes and providing solutions and future directions to further improve the efficiency of liquid chromatography columns.