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LCGC North America-12-01-2014
LCGC North America
Understanding and Improving Solid-Phase Extraction
December 01, 2014
Sample Prep Perspectives
32
12
In this installment of "Sample Prep Perspectives," we cover some of the basic scientific principles behind solid-phase extraction (SPE) to allow the correct mode of extraction to be selected through an understanding of how analytes interact with and are separated by the sorbent.
Column Protection: Three Easy Steps
December 01, 2014
LC Troubleshooting
32
12
Make your column last forever? Not quite, but you can help prevent its early demise.
Developing Better GC Methods — A Blueprint
December 01, 2014
The Essentials
32
12
To obtain sensitive, robust, and reproducible gas chromatography (GC) methods, each stage of the chromatographic process needs to be carefully considered and optimized. It is also important to record and report as much detail within the method specification so that the method can be reproduced between operators, instruments, and laboratories. Table I represents a "blueprint" method specification with all of the information that is necessary to faithfully specify and reproduce a split–splitless GC method.
Fluorescence-Detection Size-Exclusion Chromatography — An Analytical Technique with Multiple Applications
December 01, 2014
Biotechnology Today
32
12
In this installment, we discuss fluorescence-detection size exclusion chromatography (FSEC) technology and its implications in protein characterization, specifically the development and characterization of biologics.
Vol 32 No 12 LCGC North America December 2014 Regular Issue PDF
December 01, 2014
Issue PDF
32
12
Click the title above to open the LCGC North America December 2014 regular issue, Vol 32 No 12, in an interactive PDF format.
Quantification of Individual Mass Transfer Phenomena in Liquid Chromatography for Further Improvement of Column Efficiency
November 30, 2014
Peer-Reviewed Articles
32
12
This article reports on the physical phenomena that control column efficiency and on experimental protocols designed to accurately measure their contributions to band broadening of analytes during their passage from the injection to the detection device. The results of these protocols are analyzed, allowing for the accurate determination of the complete mass transfer mechanism in different separation modes and providing solutions and future directions to further improve the efficiency of liquid chromatography columns.