LCGC-North America-12-01-2020

Recombinant adeno-associated viral therapy (rAAV) products are particularly complex. Are liquid chromatography and LC–MS the right tools for their characterization?

High-capacity sorptive extraction combined with thermal desorption–gas chromatography–mass spectrometry (TD–GC–MS) can improve on traditional methods for the analysis of VOCs and other trace analytes that contribute to the aroma, flavor, and safety of food products.

Judicious initial choices of gas chromatography (GC) column dimensions, and even a change of column dimension during method development, can lead to significant improvements in resolution.

Atmospheric pressure photoionization (APPI) and electrospray ionization (ESI) are compared for the mass spectrometry (MS) quantitation of pharmaceuticals frequently detected in environmental waters, including antibiotics, beta blockers, and selective-serotonin reuptake inhibitors.

A key step in ensuring data integrity is second-person review of data, and data sets must be locked during the review process. Monica Cahilly explains.

Charged aerosol detection is a powerful complement to UV and MS, but successful implementation requires understanding a few key factors, including response dependencies on temperature, nebulization process, analyte volatility, and mobile-phase composition.