Quantitative proteomics aims to provide large-scale qualitative and quantitative data about every protein in a biological sample. However, drawbacks associated with current techniques can make it difficult to generate the data required. Research scientists have recently developed a new approach that allows highly multiplexed high-resolution MRM quantitation of most of the detectable proteins in a sample in a single analysis.
An interview with Mark Hayes, a finalist for the 2012 FACSS-SciX Innovation Award. Part of the 2013 podcast series presented in collaboration with the Federation of Analytical Chemistry and Spectroscopy Societies (FACSS), in connection with SciX 2013, the federation?s North American conference.
Wyatt Technology Corporation
Wyatt Technology Corporation
Answers to common questions about operational qualification software.
In this month's instalment, we address common questions about various gases and their delivery to a gas chromatograph.
Application Note
This article discusses the details of how the newer techniques of sample prepaation are simplified by the use of LC–MS–MS and GC–MS–MS technology.
With so many HPLC columns on the market we present a simple guide to what's important when making your stationary phase and column dimension choices.
MS: The Practical Art Editor Kate Yu interviews Fred McLafferty about his pioneering career in mass spectrometry.
A panel of experts discuss the latest trends in sample preparaion.
Small solid rubber samples can be analyzed without sample preparation.
This article describes a new analytical method for the global profiling of molecular lipids in biological samples, with particular emphasis on the plasmalogen lipids.
Fast, easy, and resproducible determination of capsaicinoid content
A sensitive method that reduces the number of false-positive results
American Chemical Society Honors Brian Bidlingmeyer
A how-to on understanding gradient elution
A systematic approach for the identification of nontargeted species using nominal and accurate mass data searching both mass spectral and "spectral-less" databases is described.
The detection limit, analytical precision, dynamic range, and robustness of a method for the targeted quantification of peptides using a capillary-flow LC–MS system were evaluated by spiking known amounts of isotopically labeled yeast peptides into a 500-ng yeast digest matrix.
An application of MALDI-MS in qualitative and quantitative analysis of pharmaceutical compounds spiked in urine is demonstrated.
The application of high-resolution TOF-MS to petroleomics is presented, and the basic theory of each type of ultrahigh-resolution mass spectral platform is briefly explained.
Leco (Michigan, USA) will support the new West Coast Metabolomics Center, based at the University of California, Davis, Genome Center (California, USA).
Should HPLC eluent additives be added to one or both of the constituent mobile phase reservoirs - both organic and aqueous, when mixing gradients online?
The American Chemical Society (ACS) has announced Brian Bidlingmeyer of Agilent Technologies, Inc. (Santa Clara, California) and a member of LCGC's editorial advisory board to the 2012 class of ACS Fellows.
Click here to read the full Europe E-newsletter from 1 March 2013.
Click the title above to open the LCGC Asia Pacific March 2013 regular issue, Vol 16, No 1, in an interactive PDF format.
Click the title above to open the LCGC Europe March 2013 regular issue, Vol 26 No 3, in an interactive PDF format.