All News

All of the most important sessions, awards and events from Pittcon 2010 covered here in this convenient and easy-to-read daily e-newsletter.

Streamlined sample prep, LC and MS method development.

The stevia plant and its extracts have long been used as sweeteners in Asia and Latin America. Two steviol glycosides present in plant tissue, stevioside and rebaudioside A, are largely responsible for the sweet flavour.1 In December 2008, the US FDA placed rebaudioside A (also known as rebiana) on the Generally Recognized as Safe (GRAS) list of sugar substitutes to be used in foods, thereby allowing the use of rebiana as a commercial sweetener.2 The determination of steviol glycosides in these sweeteners is challenging due to their weak UV absorbance. Other detection methods, such as evaporative light scattering (ELS), can improve steviol glycoside quantification. In this proposed method, steviol glycosides were determined by UV and ELS detections in consumer sweeteners following separation on the Acclaim Mixed-Mode WAX-1 column.3

Polyols are alcohols with multiple hydroxyl groups. They are used for very different applications, such as, for example, in Feed and Food as sugar substitutes or in polymer chemistry as reactants to produce high quality products.

Wednesday morning?s session, ?Achievements and Challenges in Mass Spectrometry,? was arranged by Imma Ferrer and Michael Thurman of the University of Colorado. Starting out the session will be Thurman with his opening remarks on the state of mass spectrometry.

The combination of the small particle size and narrow particle size distribution coupled with the significantly shorter diffusion path provided by the Kinetex core-shell particle results in a material that yields significantly increased column efficiencies and chromatographic resolution. This is a significant benefit for separation scientists looking to increase efficiency and chromatographic resolution, especially for complex separations containing many compounds and very closely eluting compounds so that accurate identification and quantification can be achieved.

Streamlined sample prep, LC and MS method development.

Good morning, and welcome to the final day of LCGC?s coverage from Pittcon 2010. To say the week has flown by would be an understatement, as I?m sure many conference-goers share our feeling that the conference has been a blur of activity to this point.

The 2010 Ralph N. Adams Award in Bioanalytical Chemistry will be presented to Catherine Fenselau, Professor of Chemistry and Biochemistry at the University of Maryland (College Park, Maryland), on Wednesday afternoon at Pittcon 2010.

The method developed runs on a fully automated platform integrating solid-phase extraction (SPE) and LC (Symbiosis Pico) without any manual pretreatment step(s). Automatic pretreatment results in high throughput, low CVs and high precision. Sample preparation has been simplified to just one operator step before the system takes over the automated analysis of the samples.

There is more to using and interpreting certificates for chromatography standards than meets the eye

Accurate, sensitive, and comprehensive characterization of monoclonal antibodies is an absolute necessity to the pharmaceutical and diagnostic industries to meet regulatory requirements and ensure the efficacy and safety of the final product. Microfluidic chip-based high performance liquid chromatography technology interfaced with the mass accuracy of quadrupole time-of-flight mass spectrometry provides the ability to rapidly and efficiently assess the quality of intact monoclonal antibodies, confirm their amino acid sequence, and determine their glycosylation state, while consuming very small amounts of these precious products.

The analysis of amines by gas chromatograph ;mass spectrometry (GC–MS) using electron ionization (EI) has always been a challenge

Two-dimensional gas chromatography–time-of-flight mass spectrometry (GCÃ-GC–TOF-MS) analysis has emerged as one of the technologies of choice for the analysis of small metabolite profiles. The results of these analyses produce substantial quantities of data that can be extremely time-consuming and labor-intensive for the analyst to interpret. New software provides a tool for the scientist to use as a data-mining strategy to find significant results from large, complex data sets. This proof of concept research was conducted using comprehensive GCÃ-GC–TOF-MS to elucidate the small-molecule metabolite profiles of diabetic and nondiabetic urine in search of key differences between disease-state and nondisease-state individuals.

CHROMacademy is a chromatography e-learning programme that provides continuing profesional development for chromatographers to make them more effective in the work place. There are over 3000 pages of e-learning on HPLC, GC, MS and Sample Preperation techniques to improve practical knowledge for the absolute novice (What is Chromatography?) to the expert who needs a refresher in a particular topic (Advanced Topics in LC–MS Spectral Interpretation, for example).

Which is more important: signal or noise?

The International Symposium on High Performance Liquid Phase Separations and Related Techniques (the HPLC Symposium) is one of the leading international conferences in separation science. Since the first symposium in 1973, held in Interlaken, Switzerland, the conference has focused on bringing the latest in separation science theory, technology and applications to an international audience.

All of the most important sessions, awards and events from Pittcon 2010 covered here in this convenient and easy-to-read daily e-newsletter.