Ask the Editor: Preparative GC Applications

April 10, 2008
E-Separation Solutions

Volume 0, Issue 0

What are some examples of preparative GC applications? According to R.P.W. Scott in Gas Chromatography (1), ?[t]here are a number of unique problems associated with preparative gas chromatography. Firstly, it is difficult to recycle the mobile phase and thus large volume of gas are necessary. Secondly, the sample must be fully vaporized onto the column to ensure radial distribution of the sample across the column. Thirdly, the materials of interest are eluted largely in a very dilute form from the column and therefore must be extracted or condensed from the gas stream which is also difficult to achieve efficiently.?

An LCGC reader recently submitted the following question:

What are some examples of preparative GC applications?

According to R.P.W. Scott in Gas Chromatography (1), “[t]here are a number of unique problems associated with preparative gas chromatography. Firstly, it is difficult to recycle the mobile phase and thus large volume of gas are necessary. Secondly, the sample must be fully vaporized onto the column to ensure radial distribution of the sample across the column. Thirdly, the materials of interest are eluted largely in a very dilute form from the column and therefore must be extracted or condensed from the gas stream which is also difficult to achieve efficiently.”

Preparative GC has been used for a variety of applications, including odorants in white wine (2), amino acids in tissue hydrolysates (3), polycyclic aromatic hydrocarbons (4), cockroach sex pheromone (5), and essential oil constituents (6).

(1) R.P.W. Scott, Gas Chromatography, http://www.chromatography-online.org/GC/Preparative-Gas-Chromatography/rs60.html.

(2) H. Shiratsuchi, and H. Matsusaki, Aroma Research 6(2) 188–194 (2005).

(3) K. Smith, C.M. Scrimgeour, W.M. Bennet, and M.J. Rennie, Biol. Mass Spec. 17(4), 267–273 (1988).

(4) M. Mandalakis and Ö. Gustafsson, J. Chromatography A 996(1-2), 163–172 (2003).

(5) S. Nojima, C. Schal, F.X. Webster, R.G. Santangelo, and W.L. Roelofs, Science 307(5712), 1104–1106 (2005).

(6) M. Mekem Sonwa and W.A. König, Phytochemistry 56(4), 321–326 (2001).

Questions?

LCGC technical editor Steve Brown will answer your technical questions. Each month, one question will be selected to appear in this space, so we welcome your submissions. Please send all questions to the attention of "Ask the Editor" at lcgcedit@lcgcmag.com We look forward to hearing from you.