|Articles|October 29, 2004

LCGC North America

  • LCGC North America-11-01-2004
  • Volume 22
  • Issue 11

Determining Sulfamethazine in Pork Using HPLC with a 100% Water Mobile Phase

The authors describe a method for determining sulfamethazine residue in pork using 100% water in both sample preparation and HPLC separation.

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Figure 2. Split injection used to measure triplicate test of MTBE, hexane, o-xylene, and 1-methylnaphthalene comparing different solvents (methanol, methylene chloride) and column insertion distances starting with 0.5 mm, 5.0 mm, 10.0 mm, 15 mm, and 20.0 mm. Methanol had the best performance at the 5.0 mm insertion distance and methylene chloride looked slightly better at the 0.5 mm. We would still recommend not going below the 5.0 mm manufacturer recommended insertion distance.
Revisiting insertion distance for methanol and methylene chloride in GC analysis and confirming that insertion depth strongly affects response and reproducibility.