Do 3D Prints Interfere with Untargeted LC–QTOF-MS Analyses?

Untargeted liquid chromatography–quadrupole time-of-flight mass spectrometry (LC–QTOF-MS) workflows are sensitive to background compounds leached from sample-contact materials. We develop and assess novel devices for sample preparation before chromatographic quantification using three-dimensional (3D) printing solutions, but these have only been tested for targeted analysis. In this study, we compared fused deposition modeling (FDM) and digital light processing (DLP) 3D-printed sorbent devices made from commercial and laboratory-developed materials. Our findings demonstrate that the choice of polymer and print technology critically affects blank sample complexity, and custom materials for high-sensitivity lipidomic extractions are recommended.