LCGC UST
|Articles|March 4, 2011

Column

  • The Column-03-04-2011
  • Volume 7
  • Issue 4

Drug Screening using GC×GCqMS with Thermal Modulation

In this study, the method of two-dimensional comprehensive GC applying an ultra fast quadrupole mass spectrometer as detector was used (GC×GCqMS).

High separation efficiency is the most important factor in chromatography. All further steps in chromatographic analysis methods for qualitative and quantitative data evaluation depend on it. In particular, effective separation can be achieved through two-dimensional chromatography using different separation mechanisms. In this study, the method of two-dimensional comprehensive gas chromatography (GC) applying an ultra fast quadrupole mass spectrometer as detector was used (GC×GCqMS). New quadrupole MS systems show data acquisition speeds that make them suitable for detection in comprehensive GC.

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Related to this article

Figure 2. Split injection used to measure triplicate test of MTBE, hexane, o-xylene, and 1-methylnaphthalene comparing different solvents (methanol, methylene chloride) and column insertion distances starting with 0.5 mm, 5.0 mm, 10.0 mm, 15 mm, and 20.0 mm. Methanol had the best performance at the 5.0 mm insertion distance and methylene chloride looked slightly better at the 0.5 mm. We would still recommend not going below the 5.0 mm manufacturer recommended insertion distance.
Revisiting insertion distance for methanol and methylene chloride in GC analysis and confirming that insertion depth strongly affects response and reproducibility.