How Good is Your Sample Preparation?
This article focuses on two of the more common techniques applied to the analysis of a series of benzodiazepines in a biological matrix.
Sample preparation is used routinely for bioanalysis due to the complexity of the matrix. Typically this contains a range of compounds from simple salts to large protein structures, with concentration ranges covering many orders of magnitude. Mass spectrometry is commonly used for bioanalysis, which with the discovery of ion suppression, now requires the removal of the matrix.
Several approaches can be taken to remove the matrix, including turbulent flow chromatography, solid-phase extraction (SPE), liquid–liquid extraction, protein precipitation and ultrafiltration. This article focuses on two of the more common techniques applied to the analysis of a series of benzodiazepines in a biological matrix. It studies methods to optimize SPE and also demonstrates that the use of a generic method is not always ideal due to the amount of matrix components left in the final eluant.
Essentials of LC Troubleshooting, Part VII: How Wide Should Those Peaks Be? (Large Molecule Edition)
June 5th 2025In the latest edition of "LC Troubleshooting", Dwight Stoll explores troubleshooting tips for liquid chromatography, particularly focusing on peak width challenges in large molecule separations and effective solutions.
Separating Marigold Esters Using Supercritical Chromatography
June 4th 2025Analyzing esters from marigold flowers can be a complicated process. Researchers created a new approach for separating these substances using ultra-high-performance low pressure supercritical chromatography (UHP/LP-SFC).
Modeling Chromatography Membrane Mass Transfer Using Porous Structure
June 3rd 2025To meet the increasing worldwide demand for monoclonal antibodies, it is crucial to develop purification methods that are both efficient and easily scalable. Membrane chromatography offers a promising solution for streamlining antibody purification, thanks to its ability to handle high processing volumes rapidly. Convective membrane chromatography has become popular for purifying monoclonal antibodies. Researchers created a new model for modeling mass transfer during purification.
