HTC-19 Insights: Why Combine RPLC×HILIC, cIMS, and DIA-HRMS?

At HTC-19 in Leuven, Belgium, LCGC International spoke with Nikoline Juul Nielsen from the University of Copenhagen, Denmark on her presentation ‘Data-independent Profiling of Phenolic Constituents in Plant Extracts by Comprehensive Two-dimensional Liquid Chromatography (RPLC×HILIC)-Cyclic ion Mobility-Time-of-Flight Mass Spectrometry”.¹

In this video interview Nielsen answers the following question:

• What was the rationale behind combining two-dimensional reversed-phase liquid chromatography × hydrophilic interaction liquid chromatography (RPLC × HILIC), cyclic ion mobility spectrometry (cIMS), and data-independent acquisition high-resolution mass spectrometry (DIA-HRMS) instead of using an optimized one-dimensional liquid chromatography–mass spectrometry (1D-LC–MS) workflow for the analysis of phenolic constituents in plant extracts.²

Nikoline Juul Nielsen is an Associate Professor at the Department of Plant and Environmental Science at University of Copenhagen (UCPH), Denmark. She has a BSc in Agronomy, an MSc in Environmental Chemistry and did her PhD on analytical and data processing strategies in natural products research. She specializes in fundamental and advanced analytical chemistry, with focus on multidimensional separations built on chromatography and mass spectrometry techniques. She profiles complex environmental and biological samples, and extracts information through targeted or non-targeted approaches. At UCPH, she is the head of studies for the MSc program in Environmental Science, and serves as educator and mentor, having co-founded the first Danish MSc-specialization in Analytical Chemistry, developed several specialized MSc and PhD courses and supervised graduate students and researchers. Recently, and with four colleagues, she founded the spin-out company NTS Analytica. The company assists in planning, executes and interprets customized non-target screening investigations

References

1. Nielsen N.J. Data-independent Profiling of Phenolic Constituents in Plant Extracts by Comprehensive Two-dimensional Liquid Chromatography (RPLC×HILIC)-Cyclic ion Mobility-Time-of-Flight Mass Spectrometr. Presented at HTC-19 2026, in Leuven, Belgium. https://htc-19.com/programme/( accessed 2026-06-04).
   2. Nielsen, N. J.; Kronik, O. M.; Forte Neran, R. A.; Christensen, J. H.; Ravn, T. K.; de Villiers, A. Data-Independent Profiling of Phenolic Constituents in Shea Using Comprehensive Two-Dimensional Liquid Chromatography (RPLC × HILIC) Hyphenated to Cyclic Ion Mobility–Quadrupole-Time-of-Flight Mass Spectrometry. J. Chromatogr. A 2025, 1760, 466092. DOI: https://doi.org/10.1016/j.chroma.2025.466092.