What is required to produce a highly efficient modern high performance liquid chromatography (HPLC) system/column combination? This instalment of the LCGC Blog explains more.
Tony Taylor, Crawford Scientific, Strathaven, Scotland.
Photo Credit: Dennis O’Clair/Getty Images
What is required to produce a highly efficient modern high performance liquid chromatography (HPLC) system/column combination? This instalment of the LCGC Blog explains more.
Some modern high performance liquid chromatography (HPLC) systems resemble spacecraft in terms of their technology, designed as they are to operate to the highest efficiencies, compared to traditional systems.
However, I still see countless examples where high efficiency columns are used on systems that are not matched and cannot support the highest efficiencies offered by the column.
There are many factors to consider when studying the optimum efficiency obtainable from a system/column combination. I’ve outlined some of the variables that can be easily controlled within the laboratory and those data acquisition variables which need to be considered.
For reference, I’m not going to consider capillary or nano columns here because to take advantage of these technologies you will have already considered all of the information presented here.
You will need to know the extra column volume associated with your HPLC column, which, as a first approximation, can be calculated using the information in Table 1.
The fact of the matter here is that shorter, narrower columns and those packed with highly efficient packing materials (< 2 mm or superficially porous particles) need to be used with HPLC systems whose extra column volumes are low, to avoid the system being the dominant factor in determining the maximum achievable efficiency.
The extra column volume (ECV) within a system is additive alongside the dispersion that occurs within the column in determining the peak volume (peak width); there is a very nice rule of thumb which states that the ECV of the system should be less than half of the total peak width to achieve more than 90% of the column resolving power.
Figure 1 is what we should be aiming for. The chromatographic peak volume can be estimated by drawing tangents to estimate 4σ peak width - in this case the peak width is 0.5495–0.5240 min = 0.0255 min and the flow rate was 600 μL per minute giving an estimated peak volume of 15.3 μL. This would dictate that the total system extra column volume (ECV) should be less than 8 μL (approximately) to achieve the optimum resolving power of the column/system combination.
Some typical maximum allowable ECV contributions for columns of various dimensions are shown in Table 2.
So, what do we need to take care of in order to optimize our experiments from a practical perspective?
Well, I’ve compiled a brief list below of the items that we review on each system after measuring the ECV to reconcile the measured value against what is calculated:
So - how can you estimate the ECV value?
To calculate the ACTUAL extra column volume of the system, replace the column with a zero dead volume union or capillary restrictor and inject an aliquot of 0.1% v/v acetone in water and monitor at 270 nm with your UV detector. Measure the 4 s peak width and calculate the peak volume as per Figure 1. This value is the ECV value for the system you are using.
One should carefully consider, and reduce where possible, the tubing length and internal diameter being used, especially between the column and detector, the number of unions, and any in-line filters or couplings which may not be necessary. Our earlier example with the 8 mL maximum ECV constraint would start to suffer problems if any more than 32 cm of 0.178 mm i.d. tubing is present in the whole system!
You should note the comment made earlier regarding zero contribution from ZDV unions. Well, this is only true if the connection is properly made! Ensure that the tubing is fully butted up inside the fitting and that it remains that way during the tightening operation - this is basic good practice that is often not followed. Also remember that PEEK fittings deform only so many times and old PEEK column nuts will eventually lose their ability to deform to the internal volume of the column end fitting.
Given the constraint of keeping below 8 mL ECV, one may also need to consider for our system, the required injection volume. Clearly injection volumes need to be scaled when using lower volume columns and a nice estimate of maximum injection volume is around 15% of the peak volume. So restricting injection volume to no more than 2.25 mL would be sensible for our example here. If scaling down a method to a smaller column dimension, and assuming a constant particle porosity, then a simple scaling relationship is:
Vinj (2) = Vinj (1) × (i.d.(1) × L(1) / i.d.(2) × L(2)) [1]
where Vinj (1) is original injection volume, i.d.(1) the internal diameter of the original column, and so forth…
Another very useful rule of thumb in reducing ECV and minimizing peak dispersion to realize maximum efficiency is to restrict the flow cell of the detector to less than 10% of the calculated peak volume. In our previous example the peak volume was approximately 15 μL, which indicates a maximum flow cell volume of around 1.5 μL. This indicates the need for a flow cell based on light pipe principles, which have very low internal volume whilst still maintaining good sensitivity.
Table 4 outlines the relative merits of flow cells of various dimensions.
The most surprising contributor to peak dispersion is the data acquisition parameters that we apply to a method. The data sampling rate can have a direct effect on peak width (efficiency) and as such these are important parameters that must be matched to the widths of peaks generated by various column dimensions.
However, data sampling rate is not the only important factor in determining peak width and variables such as bunch rate or time constant, that is, the digital bunching and filtering applied by the detector, are also of importance and some guideline as to these variables are shown in Table 5.
To conclude, Figure 3 shows the effect of extra column volume on a chromatographic separation using a column of 100 × 2.1 mm with an ECV of 20 μL (bottom) and 80 μL (top).
It should be clear from Figure 3 that the upper separation would not find favour in regular use and that the efficiency of peak 7 has fallen by more than half due to a 4× increase in system extra column volume.
It is very important when using reduced dimension HPLC columns and high efficiency particle morphologies that the system volume and data acquisition parameters are optimized to realize the full efficiency benefits.
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