On-line Highlights

This week on Chromatography Online, leading experts shared practical insights with LCGC International on sustainable affinity methods, siRNA impurity profiling, nontarget screening, peptide tech transfer, and AI in separation science.

Liquid chromatography–mass spectrometry (LC–MS) was used to determine if there is a link between artificial sweeteners and breast cancer.

Chiral gas chromatography–mass spectrometry (GC–MS) shows how heat and storage alter linalool in blueberry juice.

Anneli Kruve reports that toxicity predictions from MS2-derived molecular fingerprints outperform spectral-similarity methods in nontarget screening of food.

In this fourth part of " Conversations with Michael Dong," a series on fundamentals of HPLC in pharmaceutical analysis, LCGC International’s "Perspectives in HPLC" column editor explains why buffers are most often used in Mobile Phase A in high-pressure liquid chromatography of pharmaceuticals.

Skipping solid-phase extraction (SPE) cleanup rarely hurt liquid chromatography–tandem mass spectrometry (LC–MS/MS) plant hormone results in tomato.

The panel considers how AI and automation could reshape peptide method development, and why scientific judgment will remain essential.

Gas chromatography–mass spectrometry (GC–MS) and docking reveal A. excelsa leaf compounds that may help in cancer treatment.