Proteomics LC/MS: How to Maximize Sensitivity, Depth of Analysis, Throughput and Robustness

Discover how a new LC/MS system brings unmatched synergy, sensitivity, and robustness to bottom-up proteomics. This webinar reveals how optimizing both LC and MS—not just one—can transform your peptide analysis.

Register Free: https://www.chromatographyonline.com/lcgc_w/proteomics-lcms

Event Overview:

In bottom-up proteomics, the key is how to efficiently separate extremely complex peptide samples that contain more than a million types of peptides in a concentration range of more than six orders of magnitude. For this purpose, nano-scale liquid chromatography (LC) and tandem mass spectrometry (MS) are used, in which precursor ions are separated by both reverse-phase LC using an acidic mobile phase and a front-mass analyzer such as a quadrupole. Then, collision-induced dissociation is used to generate the series of product ions necessary for peptide identification. This webinar will discuss the optimal proteomics LC/MS that produces a synergistic effect between LC and MS, in contrast to chromatographers who only think of MS as a detector and mass spectrometrists who try to solve everything with MS alone. It will introduce a new system that achieves a balance between depth of analysis, sensitivity and throughput, and lastly, system robustness, which is always a problem in actual analysis.

Key Learning Objectives

• Learn how LC and MS work for efficient peptide separation in proteomics.
• Discover a system that balances analysis depth, sensitivity, and throughput.
• Understand strategies to improve system robustness in proteomics analysis.
  

Who Should Attend

• Professionals in proteomics, LC/MS specialists, and analytical chemists.
• Researchers, lab managers, and those interested in advanced LC/MS techniques.

Featured Speaker:

Yasushi Ishihama
Professor
Kyoto University

Yasushi Ishihama has been a Professor of Graduate School of Pharmaceutical Sciences, Kyoto University since 2010. He has not only developed basic proteomics technologies but has also contributed to this field by applying developed technologies to biology. He received the Award of Japanese Proteomics Society (2013), the Award of the Society for Chromatographic Sciences (2018), and the Award of the Japan Society of Analytical Chemistry (2021).

Register Free: https://www.chromatographyonline.com/lcgc_w/proteomics-lcms