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The Application Notebook
Xiaoyan Wang, UCT, LLC
Benzodiazepines are psychoactive drugs widely prescribed for treating anxiety, insomnia, agitation, seizures, muscle spasms, and alcohol withdrawal. Common sample preparation methods to monitor for this drug class in whole blood include a protein precipitation step followed by either a time-consuming
liquid–liquid extraction (LLE) or solid-phase extraction (SPE) procedure. This featured application note describes an easy, fast, and effective method using the QuEChERS approach to provide for their quantitative analysis.
LC–MS–MS: Agilent 1200 Binary Pump SL / AB Sciex API 4000 Qtrap MS–MS
Column: 100 × 2.1 mm, 3-μm
UCT Selectra® DA LC column,
Guard column: 10 × 2.1 mm, 3-μm
UCT Selectra® DA guard column,
Injection volume: 10 μL
Mobile phase A: 0.1% formic acid in D.I. H2O
Mobile phase B: 0.1% formic acid in methanol
Column flow rate: 0.30 mL/min
Excellent recoveries (85.5–105%) and relative standard deviations (RSD% ≤ 10.7%) were achieved. Matrix effects were found to be minor, ranging from -22% to 18%. This indicates that the QuEChERS technique with dSPE cleanup quickly and effectively removes unwanted matrix interferences that may cause significant ion suppression or enhancement.
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