Rapid Analysis of Corn Stover Acid Hydrolysate for Estimation of Total Monosaccharide Content
Corn stover is the above-ground portion of the plant minus the kernels and it accounts for a large percentage of the global supply of lignocelluosic biomass available as feedstock for fermentation systems used for biofuel production.
Corn stover is the above-ground portion of the plant minus the kernels and it accounts for a large percentage of the global supply of lignocelluosic biomass available as feedstock for fermentation systems used for biofuel production. Acid hydrolysis of corn stover with sulfuric acid is a common method to release water-soluble carbohydrates for biofuel production. Monosaccharide concentrations released can vary with the feedstock, its pretreatment, hydrolysis, and storage conditions. Knowledge of the monosaccharide content of acid-hydrolyzed corn stover allows evaluation of the effectiveness of a new hydrolysis process and/or an estimation of the product yield.
Carbohydrates lack a good chromophore and therefore require high concentrations to be detected by UV absorbance or refractive index. Non-carbohydrate ingredients of acid-hydrolyzed corn stover with good chromophoric properties can interfere with carbohydrate determinations. Pulsed amperometric detection (PAD) is selective for compounds that can be detected under a given set of electrochemical conditions, and has a wide linear range for carbohydrates.
High-performance anion-exchange chromatography (HPAE) can separate glucose, galactose, arabinose, xylose, mannose, fructose, cellobiose, rhamnose, sucrose, mannitol, and other carbohydrates typical of acid-hydrolyzed plant-derived materials. The CarboPac® PA1 anion-exchange column resolves monosaccharides and disaccharides from the unretained non-carbohydrate components using hydroxide eluent. Generally, at high hydroxide concentrations (e.g., 200 mM NaOH), monosaccharides elute rapidly and are poorly resolved. When the knowledge of the types of monosaccharides present in the acid-hydrolyzed feed stock is not necessary, a rapid method that combines the total monosaccharides into one or two peaks for quantification may be more desirable (1). The method presented here allows estimation of total monosaccharides in undiluted corn stover acid hydrolysate in less than 10 min using HPAE-PAD.
Experimental
Dionex ICS-3000 chromatography system consisting of a pump, detector chromatography module (with electrochemical detector, gold working electrode with 15 ml PTFE gasket, 0.2 μL injection loop), and autosampler. The CarboPac PA1 column was used with 200 mM NaOH at 30 °C with a flow rate of 1 mL/min. Corn stover acid hydrolysate was centrifuged at 16k × g for 10 min to remove particulates, then directly injected (1).
Results and Discussion
Figure 1 shows the chromatograms of undiluted corn stover hydrolysate (blue trace) and standards (magenta trace). Panel B reveals greater detail. Arabinose (peak 1) is resolved from galactose, glucose, mannose, and xylose (peak 2), and fructose (peak 3). The combined peak area for peaks 1, 2, and 3 is an estimate of the monosaccharide content of this sample.
Figure 1
Reference
(1) Dionex Corporation. Rapid Method for the Estimation of Total Free Monosaccharide Content in Corn Stover Hydrolysate Using HPAE-PAD. Application Note 225, LPN 2190. Sunnyvale, CA.
CarboPac is a registered trademark of Dionex Corporation.
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