Allthough GPC/SEC columns are expected to be, in general, less stable than HPLC columns, they can be used for several years if handled properly.
Unlike HPLC columns, GPC/SEC columns are made of crosslinked polymeric materials with a permanent porosity. Because of the permanent porosity GPC/SEC columns are pressure sensitive and need more care than other LC columns.
An important quality criterion for the lifetime of columns is the crosslinking density. A low crosslinking density leads to poor mechanical properties and low solvent compatibility. However, highly crosslinked material supports unwanted adsorption, so that a good compromise needs to be found.
Packing of GPC/SEC columns requires special knowledge and strict procedures to achieve highest resolution and optimum size separation. This makes GPC/SEC columns more expensive than HPLC ones.
Allthough GPC/SEC columns are expected to be, in general, less stable than HPLC columns, they can be used for several years if handled properly.
Inside the Laboratory: The Gionfriddo Group at the University at Buffalo
March 28th 2024In this edition of “Inside the Laboratory,” Emanuela Gionfriddo, PhD, an associate professor of chemistry at the University at Buffalo, discusses her group’s current research endeavors, including using solid-phase microextraction (SPME) coupled to liquid chromatography (LC) and gas chromatography (GC) to further understand the chemical relationship between environmental exposure and disease and elucidate micropollutants fate in the environment and biological systems.
Transferring Methods to Compact and Portable HPLC
February 14th 2024The current trend in laboratory equipment design is the miniaturization of laboratory instruments. Smaller-scale HPLC instruments offer benefits that cannot be matched by analytical-scale equipment, especially in the areas of portability, reduced fluid volumes, and reduced operating costs. Yet, the miniaturization of laboratory equipment has brought with it a unique set of challenges, including transferring methods to compact LC. Capillary LC expands the use of LC to applications not currently done using conventional LC in a wide array of application areas, including pharmaceutical, food and beverage, petrochemical, environmental, and oil and gas. Greg Ward, Axcend’s CEO wrote, “Customers want an HPLC system with a small footprint, low flow rates and green chemistry.” Join his podcast where he shares method transfer in these application areas.