Tips & Tricks: GPC/SEC How to Expand The Lifetime of Columns

Unlike HPLC columns, GPC/SEC columns are made of crosslinked polymeric materials with a permanent porosity. Because of the permanent porosity GPC/SEC columns are pressure sensitive and need more care than other LC columns.
An important quality criterion for the lifetime of columns is the crosslinking density. A low crosslinking density leads to poor mechanical properties and low solvent compatibility. However, highly crosslinked material supports unwanted adsorption, so that a good compromise needs to be found.
Packing of GPC/SEC columns requires special knowledge and strict procedures to achieve highest resolution and optimum size separation. This makes GPC/SEC columns more expensive than HPLC ones.
Allthough GPC/SEC columns are expected to be, in general, less stable than HPLC columns, they can be used for several years if handled properly.