Two Dimensional Liquid Chromatography: A Powerful Tool for The Characterization of Humic Substances

Analysis of natural compounds is becoming increasingly important. One thriving area is the evaluation and application of environmental biopolymers, such as humic substances (HSs) in various applications, including medicine, industry, agriculture and environmental protection. Such work is aided by ancient knowledge on the biological activity of HSs and their interactions with other living organisms. HSs — along with cellulose and chitin — are some of the most common types of organic matter on Earth.