GPC/SEC Tips & Tricks: Improving the Reliability of GPC/SEC Light-Scattering and Viscometry Results
On-line light scattering and viscometer detectors increase the information depth of GPC/SEC data and allow absolute molar masses and structures to be measured.
On-line light scattering and viscometer detectors increase the information depth of GPC/SEC data and allow absolute molar masses and structures to be measured. However, even a set-up with advanced instrumentation is not parameter free and the inter-lab comparison of results is often sobering. Sometimes even the comparison of inter-lab results or the reproducibility is not acceptable. In most instances the reason for these deviations can be related to a wrong determination of the slice concentration. In contrast to conventional GPC/SEC, hyphenation with molar mass sensitive detection requires the precise and accurate knowledge of the mass in every chromatographic slice
LC–MS/MS-Based System Used to Profile Ceramide Reactions to Diseases
April 26th 2024Scientists from the University of Córdoba in Córdoba, Spain recently used liquid chromatography–tandem mass spectrometry (LC–MS/MS) to comprehensively profile human ceramides to determine their reactions to diseases.
High-Throughput 4D TIMS Method Accelerates Lipidomics Analysis
April 25th 2024Ultrahigh-pressure liquid chromatography coupled to high-resolution mass spectrometry (UHPLC-HRMS) had been previously proposed for untargeted lipidomics analysis, but this updated approach was reported by the authors to reduce run time to 4 min.
