PharmTech LCGC Sciex Webinar 11.10
|Articles|October 17, 2011

Column

  • The Column-10-17-2011
  • Volume 7
  • Issue 19

GPC/SEC Tips & Tricks: Improving the Reliability of GPC/SEC Light-Scattering and Viscometry Results

On-line light scattering and viscometer detectors increase the information depth of GPC/SEC data and allow absolute molar masses and structures to be measured.

On-line light scattering and viscometer detectors increase the information depth of GPC/SEC data and allow absolute molar masses and structures to be measured. However, even a set-up with advanced instrumentation is not parameter free and the inter-lab comparison of results is often sobering. Sometimes even the comparison of inter-lab results or the reproducibility is not acceptable. In most instances the reason for these deviations can be related to a wrong determination of the slice concentration. In contrast to conventional GPC/SEC, hyphenation with molar mass sensitive detection requires the precise and accurate knowledge of the mass in every chromatographic slice


Related to this article

Best of the Week: From Cancer Diagnostics to Smarter LC Method Development
This week, Chromatography Online’s coverage spanned lipidomics-based early cancer detection, career paths in separation science, machine learning and uncertainty-driven approaches to LC method development, new EU wastewater rules for pharmaceutical micropollutants, automated Separation Quality Factor (SQF) evaluation in chromatography data systems, and solutions to GLP-1 peptide analysis challenges.