|Articles|April 1, 2002

LCGC North America

  • LCGC North America-04-01-2002
  • Volume 20
  • Issue 4

Uncovering Deficiencies in Mass Balance Using HPLC with Chemiluminescence Nitrogen-Specific Detection

The author describes using HPLC with chemiluminescence nitrogen-specific detection and aperometric detection to determine organic impurities in a tedisamil dihydrochloride drug substance.


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Chromatography Detects Toxins in Tea Samples
High-performance liquid chromatography-tandem mass spectroscopy (HPLC–MS/MS) chromatography reveals pyrrolizidine alkaloids (PA)/pyrrolizidine alkaloid N-oxides (PANO) toxin risks in 63 tea samples.
Figure 2. Split injection used to measure triplicate test of MTBE, hexane, o-xylene, and 1-methylnaphthalene comparing different solvents (methanol, methylene chloride) and column insertion distances starting with 0.5 mm, 5.0 mm, 10.0 mm, 15 mm, and 20.0 mm. Methanol had the best performance at the 5.0 mm insertion distance and methylene chloride looked slightly better at the 0.5 mm. We would still recommend not going below the 5.0 mm manufacturer recommended insertion distance.
Revisiting insertion distance for methanol and methylene chloride in GC analysis and confirming that insertion depth strongly affects response and reproducibility.