What’s Light Got to Do with It? How Light Scattering Relates to Molecular Weight and Size of Biomolecules and Polymers

The need for enhanced product quality of biomolecules has led to a higher-level of characterization of different critical quality attributes. Size exclusion chromatography (SEC) has been widely used as a technique to provide size and purity. SEC coupled with multi-angle light scattering (MALS) can determine molecular weight (MW) and size (or radius of gyration, Rg) of complex samples. Dr. Wei Lu, Ph.D. and Philip Hoang from Tosoh Bioscience discuss how SEC-MALS as an analytical method can be optimized and applied to a wide range of applications including oligonucleotides, monoclonal antibodies, and virus-like particles.