FoodOmicsGR_RI aims to characterize unique Greek products and highlight their value and quality using cutting-edge omics technology.
Analysis of the compositional variation in living cells is essential for understanding biological processes. Single-cell elemental analysis by triple-quadrupole ICP-MS is emerging as a selective, highly sensitive, and potentially high-throughput technique for the study of constitutive elements, and uptake of metallodrugs (or metal-containing nanomaterials) in single cells.
Determination of oligomeric states is an important issue in protein chemistry. For example, self-assembly via oligomerization domains is crucial for the regulation of several protein kinases. Determination of the oligomeric state of fragments of these kinases is a means of verifying the involvement of each domain in self-assembly.
This review article summarizes the results obtained from the combined efforts of a joint academic and industrial initiative to solve the real-life challenge of determining low levels of peptide-related impurities in the presence of the related biologically-active peptide at a high concentration.
Lipids in SFC are mainly separated according to their lipid classes, which simplifies quantification with standards. In this study, SFC coupled with drift time IM‑QTOF-MS was used to characterize the HepG2 lipidome.
Discover a reliable and robust HPLC method to separate Δ10-THC and Δ6a,10a-THC isomers produced from Δ9-THC degradation using a chiral HPLC stationary phase.
Nonspecific adsorption leads to poor peak shapes and poor performance from HPLC systems, but coating-enabling technologies can serve as a potential solution. We explain how.
A column with chemically modified column hardware showed improvements in analytical performance for siRNA compared to a conventional stainless-steel column.
This article reviews a range of techniques available for separative analysis of mRNA therapeutics, their associated impurities, and delivery vehicles.
Pyrolysis–gas chromatography–mass spectrometry has advantages for the analysis of environmental microplastic samples compared to other leading analytical methods, including spectroscopic techniques.
HRIM has emerged as a robust separation strategy for complex chemical analyses due to its ability to improve peak capacity and aid in the separation of isobaric signals.
The application of porous layer open tubular (PLOT) columns can be expanded beyond light compounds to separations of analytes with heavier boiling points.
Microextraction techniques have evolved since their appearance three decades ago, moving toward the use of greener and sustainable materials and solvents.
Untargeted GC–MS metabolomics with sample derivatization is shown here to be effective for measuring the chemical profiles of traditional and plant-based meat products.
Gene therapy vectors in the gigadalton (GDa) regime are beyond the range of conventional mass spectrometry. Charge-detection MS overcomes this limitation.
Decomposing animal tissue releases volatile organic compounds (VOCs), of interest in forensic science. We describe the use of GC×GC–qMS/FID retrofitted with a reverse fill/flush (RFF) flow modulator for analyzing these VOCs in a tropical climate.
We present the main analytical techniques for performing functional characterization of biotherapeutic products. Such assessments are particularly critical for biosimilars, where analytical testing must ensure functional comparability with the innovator product.
A focus on untargeted UHPLC–MS/MS lipidomics with data independent acquisition using SWATH technology. This article explores the wide-ranging potential and peculiarities of SWATH–MS hyphenated with UHPLC for the profiling of lipidomes of various biological samples.
Ambient air analysis and study of the new IONICON Fusion PTR-TOF, providing ultrahigh sensitivities up to 80 000 cps/ppbV, high mass resolving power and ppqV detection limit
Learn how to use flash chromatography and prep HPLC for protein purification, parameters that improve resolution, and how to select the stationary phase and solvent.
In electromembrane extraction (EME), the target analyte is extracted from an aqueous sample across a supported liquid membrane (SLM) and analyzed using LC. Through the study presented here, we demonstrate the principles of the technique and test its performance.
A new automated active pharmaceutical ingredient (API) extraction approach with integrated analysis by online liquid chromatography (LC) to assess drug product potency is presented.
A novel analysis technique has been developed for the determination of microplastics (MPs) in complex environmental samples using thermal extraction desorption gas chromatography–mass spectrometry (TED-GC–MS).
An analytical method for the separation of oligonucleotides of different chain length by IEC using a bio-inert UHPLC system is presented.
Acoustic ejection mass spectrometry (AEMS) has recently emerged as the premier ultrahigh-throughput mass spectrometric methodology for drug discovery and related fields.
An improved LC–MS/MS method that can rapidly detect fipronil, an insecticide harmful to human health, is presented here for chicken eggs, feed, and soil.
October 4, 2021 at 3pm BST | 10am EST | 4pm CEST Join global industry and regulatory experts as they discuss current hot topics in extractables and leachables (E&L) testing for pharmaceuticals, biopharmaceuticals and medical devices.
This instalment of Tips & Tricks focuses on whether a complete separation can be achieved using GPC/SEC and, if so, under what conditions.