Pressure-enhanced liquid chromatography (PE-LC) offers a new approach for improving selectivity for large molecule separations. Examples shown here include short oligonucleotides in ion-pairing reversed-phase (IP-RP) liquid chromatography and larger nucleic acids in ion-exchange (IEX) chromatography.
During the past five years, many manufacturers of HPLC columns have focused on improving stationary phase stability and reproducibility. In this study, the authors use a variety of test solutes to compare the efficiency, selectivity, and hydrophobic retention mechanisms of five commercially available HPLC columns based silica, alumina, zirconia, and polystyrene cross-linked with divinylbenzene as the support.
The values determined by van’t Hoff analysis only have practical significance if we analyze the results obtained with well-planned and systematically varied experiments.
When it comes to high-purity inorganic raw materials, the key to analytical success is knowing how to define purity. A variety of factors can impact this definition, including the specific application as well as the chemist’s needs.
An overview of different approaches for the qualitative and quantitative analysis of polysorbates.
Further miniaturization of separations will greatly extend the reach of single-cell proteomics, metabolomics, and lipidomics, but key challenges in instrumentation, column technology, and ionization sources must be addressed.
A new automated active pharmaceutical ingredient (API) extraction approach with integrated analysis by online liquid chromatography (LC) to assess drug product potency is presented.
Decomposing animal tissue releases volatile organic compounds (VOCs), of interest in forensic science. We describe the use of GC×GC–qMS/FID retrofitted with a reverse fill/flush (RFF) flow modulator for analyzing these VOCs in a tropical climate.
In this extended special feature to celebrate the 35th anniversary edition of LCGC Europe, leading figures from the separation science community explore contemporary trends in separation science and identify possible future developments.
This application note demonstrates a novel composite technology that enhances reproducibility of solid phase extraction compared to a traditional loose packed format.
In our study, a fast and sensitive liquid chromatography coupled to tandem mass spectrometry (LC–MS/MS) method for the determination of vitamin D2 in fresh mushrooms and its metabolite 25(OH)D2 in the blood of volunteers regularly consuming UV-treated mushrooms has been introduced.
This article describes the method development involved in the authentication of nutraceuticals, particularly those containing Panax ginseng, which is popular because of possible positive effects on human health.
This simple, rapid, and accurate HPLC-UV method can facilitate routine therapeutic drug monitoring in neonates.
This application note demonstrates how the Empore EZ-Trace SPE system is used to perform multiple extractions simultaneously while achieving clean and reproducible results using EPA Method 525.3 as an example application.
Matrix interferences can impact routine analysis with triple quadrupole methods for monitoring and quantifying PFAS in food.
This review article discusses scientific rationales and current best practices in the pharmaceutical industry for performing chromatographic peak purity assessments (PPA). These activities are associated with the development and validation of liquid chromatographic (LC) stability-indicating analytical methods applicable to regulatory submissions of small-molecule drug candidates. The discussion includes a comprehensive overview of the PPA-related regulatory and scientific landscape and common industry approaches to obtain PPA results, as well as the strengths and weaknesses of PDA-facilitated ultraviolet (UV) PPA and other PPA techniques.
This article presents a new hydrophilic SAX hybrid monolithic column prepared by in‑capillary coating 5 μm bare silica particles with functional organic polymers. After preparation conditions were investigated and selected, the resulting column was characterized in detail.
FoodOmicsGR_RI aims to characterize unique Greek products and highlight their value and quality using cutting-edge omics technology.
An overview of different approaches for the qualitative and quantitative analysis of polysorbates.
The analysis of contaminants found in environmental waters and originating from personal care products using metalorganic frameworks (MOFs) combined with liquid chromatography (LC) is described. This work expands the use of MOFs from gas chromatography to LC and also meets the requirements of green analytical chemistry.
HS-GC analysis is presented as an excellent method for the analysis of high volatile components in e-liquids. For the analysis of semivolatile ingredients, an additional sample preparation step is proposed based on a LLE followed by a freeze-out of the matrix components.
FoodOmicsGR_RI aims to characterize unique Greek products and highlight their value and quality using cutting-edge omics technology.
The identification and quantification of sulphur are real analytical challenges, demanding selective and sensitive methods. Analysis of products such as beer, wine, coffee, and vegetables are discussed.
Microextraction techniques have evolved since their appearance three decades ago, moving toward the use of greener and sustainable materials and solvents.
GLP helps ensure the quality of different studies during drug development. Here, the authors outline practices relevant to analytical and bioanalytical chemists.
A micro-pillar array format for mapping the proteome of human stem cell-derived liver organoids using timsTOF–MS is presented.