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The Application Notebook-07-01-2018

The Application Notebook

Fast and Sensitive Analysis of Amino Acids Using Automated Pre-Column Derivatization

July 01, 2018

Food and Beverage

33

2

The building blocks of peptides and proteins, amino acids, are present in animals, humans, and plants. The analysis of amino acids is of essential significance in several areas including food science, clinical diagnostics, and pharmaceutical products research. Classical liquid chromatography (LC) determination of amino acids is performed with sample pretreatment including pre-column as well as post-column derivatization to improve or enable spectroscopic detection.

In-Well Hydrolysis Using UCT’s Refine™ Ultra-Filtration Technology

July 01, 2018

Medical/Biological

33

2

UCT’s Refine™ Ultra-Filtration Technology allows for sample precipitation and filtration to occur simultaneously in the individual column or well. A novel, hydrophobically treated, submicrometer frit combination facilitates the removal of sample proteins without a complicated extraction.

Analysis of Fentanyl and Its Analogues in Human Urine by LC–MS/MS

July 01, 2018

Medical/Biological

33

2

Abuse of synthetic opioid prescription painkillers such as fentanyl, along with a rapidly growing list of illicit analogues, is a significant public health problem. In this study, we developed a simple dilute-and-shoot method that provides a fast 3.5-min analysis of fentanyl and related compounds (norfentanyl, acetyl fentanyl, alfentanil, butyryl fentanyl, carfentanil, remifentanil, and sufentanil) in human urine by LC–MS/MS using a Raptor Biphenyl column.

Drug-Antibody Ratio of an Antibody–Drug Conjugate by SEC-MALS

July 01, 2018

Pharmaceutical/Drug Discovery

33

2

Drug-antibody ratio (DAR) is a critical attribute of antibody–drug conjugates (ADCs). DAR may often be determined quickly and effectively by combining SEC with three online detectors: MALS, UV, and dRI.