January 2022

Aspects of applying AF4 to plasma, serum, milk, and cerebrospinal fluid in the field of analysis and characterization of proteins, biologics, and nanoparticles in biological fluids are reviewed.

Some “LC Troubleshooting” topics never get old because there are some problems that persist in the practice of LC, even as instrument technology improves over time.

In this instalment we take a close look at Golay’s famous equation that most people see as relating HETP (height equivalent to a theoretical plate) to the carrier gas flow rate or average linear gas velocity in a capillary column.

QuEChERS has recently been updated to QuEChERSER (“more than QuEChERS” or also “efficient and robust”) to better take advantage of the features provided by modern MS-based detection tools.

A rapid LC–MS method using ESI coupled with SIM-MS for analysis of humulone and isohumulone content in beer is described.