LCGC Europe-05-01-2005

This month, John Dolan turns his attention to the unexplained forces that seem to inhabit laboratories in spite of the most ardent troubleshooting efforts.

This month’s column provides an overview of green chemistry issues relating to preparative chiral SFC chromatography in support of preclinical development in the pharmaceutical industry.

Bob McDowall stresses the importance of using accurate and precise time and date stamps to ensure the integrity of the data and results generated by computerized systems in any chromatography laboratory.

John Hinshaw asks what constitutes a chromatographic method and addresses some of the issues that method transfer raises for method documentation and validation.

The authors describe how this free downloadable software calculates and displays CZE separations after the user has entered sample and operations parameters. The underlying principles and mathematical basis are explained.

This article examines the results of chromatographic performance when using different buffers. A study of the origins of the differences in peak shape obtained when using varying buffers–additives is presented with regard to the analysis of basic peptides in reversed-phase HPLC.