LCGC North America-02-01-2020

Click the title above to open the LCGC North America February 2020 issue, Volume 38, Number 2, in an interactive PDF format.

In situ measurements of the mobile-phase pH before and after the column help to rationalize the effects of mismatch in pH and concentration between the mobile phase and sample buffer mismatch in reversed-phase LC separations.

At the 43rd International Symposium on Capillary Chromatography (ISCC 2019) in Fort Worth, Texas, a panel addressed the current challenges and potential future directions in capillary liquid chromatography. How can their vision be achieved?

A top-down approach to quantifying intact proteins from biological fluids using liquid chromatography–triple quadrupole–mass spectrometry (LC–MS/MS) offers the potential for more absolute quantitation than bottom-up approaches. To make this broadly viable, however, commercial sample preparation tools are needed.

Hybrid chromatography data systems are the worst possible solution for any laboratory, whether regulated or unregulated, and yet they are pervasive. Here’s why.

Daniel W. Armstrong and Szabolcs Fekete are the winners of the 13th annual LCGC Lifetime Achievement and Emerging Leader in Chromatography Awards, respectively. Here, we review their achievements.

A method was developed for the molecular weight characterization of heterogeneous polymer mixtures, such as heparins and glatiramer acetate, noting that single molecular structures are not adequate for creating a molecular weight calibration curve. That limitation is overcome in this work, which demonstrates method validation and application to process samples.