LCGC North America-02-01-2020

LCGC North America

Vol 38 No 2 LCGC North America February 2020 Regular Issue PDF

February 01, 2020

LCGC North America February 2020

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Click the title above to open the LCGC North America February 2020 issue, Volume 38, Number 2, in an interactive PDF format.

Effects of Buffer Capacity in LC, Part II: Visualization of pH Changes Inside the Column

February 01, 2020

LC Troubleshooting

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In situ measurements of the mobile-phase pH before and after the column help to rationalize the effects of mismatch in pH and concentration between the mobile phase and sample buffer mismatch in reversed-phase LC separations.

State-of-the-Art in Capillary Liquid Chromatography (LC): Now, Next, and How?

February 01, 2020

Column Watch

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At the 43rd International Symposium on Capillary Chromatography (ISCC 2019) in Fort Worth, Texas, a panel addressed the current challenges and potential future directions in capillary liquid chromatography. How can their vision be achieved?

Commercial Sample Preparation Materials for Isolation of Intact Proteins from Biological Samples are Absent in the Marketplace

February 01, 2020

Viewpoints

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A top-down approach to quantifying intact proteins from biological fluids using liquid chromatography–triple quadrupole–mass spectrometry (LC–MS/MS) offers the potential for more absolute quantitation than bottom-up approaches. To make this broadly viable, however, commercial sample preparation tools are needed.

Molecular Weight Analysis of Glatiramer Acetate and Related Compounds by Size-Exclusion Chromatography with Multi-Angle Light Scattering Detection (SEC-MALS)

February 01, 2020

Peer-Reviewed article

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A method was developed for the molecular weight characterization of heterogeneous polymer mixtures, such as heparins and glatiramer acetate, noting that single molecular structures are not adequate for creating a molecular weight calibration curve. That limitation is overcome in this work, which demonstrates method validation and application to process samples.