LCGC North America-03-01-2003

This article reviews several common applications of the critical-pair approach to column chromatography as it is applied under pharmaceutical guidelines and validation strategies.

John Hinshaw examines the effects of system time constants and sampling rates on apparent peak shapes and areas.

The first of two columns about new chromatography columns, accessories, and sample preparation products introduced at Pittcon 2003.

Two solutions to the problem of obtaining quantitative information about protein expression are to couple two or more chromatographic modes to increase resolution and to use affinity selection techniques.

John Dolan provides 10 tips for the proper care and use of LC columns.