LCGC North America-11-01-2019

Click the title above to open the LCGC North America November 2019 issue, Volume 37, Number 11, in an interactive PDF format.

Many users have questions about how long LC columns should be re-equilibrated following solvent gradient elution separations. Here we show satisfactory results with short re-equilibration periods, for both reversed-phase and HILIC separations.

LIMS are software platforms that provide data management, and were introduced as a solution to sample tracking. However, LIMS quickly evolved into a tool that manages multiple facets of laboratory informatics.

Hydrogen–deuterium exchange–mass spectrometry enables determination of higher order structures of biopharmaceuticals and direct comparisons between a biosimilar and its proprietary analog.

Jim Garvey of the Pesticide Control Laboratory in Ireland discusses the development of pesticide analysis methods with high-resolution accurate mass MS.

The effectiveness of a separation can be quantified by measuring the resolution. This article explains the factors influencing resolution and the equation for predicting the required number of theoretical plates to obtain a given esolution.

These three tips provide a clear starting point developing any HPLC method, to achieve optimum resolution in the minimum amount of time.