Many users have questions about how long LC columns should be re-equilibrated following solvent gradient elution separations. Here we show satisfactory results with short re-equilibration periods, for both reversed-phase and HILIC separations.
LIMS are software platforms that provide data management, and were introduced as a solution to sample tracking. However, LIMS quickly evolved into a tool that manages multiple facets of laboratory informatics.
The effectiveness of a separation can be quantified by measuring the resolution. This article explains the factors influencing resolution and the equation for predicting the required number of theoretical plates to obtain a given esolution.