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Special Issues-11-01-2019 | LCGC Supplements
Special Issues
Vol 37, s11 Advances in Biopharmaceutical Analysis
November 01, 2019
Special Issues November 2019
37
11
Click the title above to open the Advances inBiopharmaceutical Analysis 2019 special issue in an interactive PDF format.
Considerations and Advances in Developing Analytical Assays for Measuring Product-Related Variants in Bispecific Antibodies
November 01, 2019
Articles
37
11
Given the complexity of producing bispecific antibodies, suitable analytical methods to detect and measure the levels of undesired variants are essential. Here, a novel MS-based method for variant detection is presented.
Advancing Biopharmaceutical Analysis
November 01, 2019
Articles
37
11
An introduction to this special issue by our guest editor
Monitoring of On-column Methionine Oxidation as Part of a System Suitability Test During UHPLC–MS/MS Peptide Mapping
November 01, 2019
Articles
37
11
Long-term column use can lead to on-column methionine oxidation during LC–MS/MS peptide mapping of antibody-based biotherapeutics. Following the approach described here minimizes the risk of measuring oxidative artifacts, and helps generate high quality data to provide reliable quantitative information about product-related heterogeneities.
Size-Exclusion Chromatography for Analyzing Complex and Novel Biotherapeutic Products
November 01, 2019
Articles
37
11
Applications of size-exclusion chromatography (SEC) are presented for characterization and quality control of novel biotherapeutic products, including antibody–drug conjugates, hydrophobic proteins, and coformulations.
Ferritin as a Natural Protein Scaffold: Building a Multivalent Ferritin–Fab Conjugate
November 01, 2019
Articles
37
11
In this study, a conjugation strategy for Fab–ferritin conjugates, used for drug delivery, was successfully optimized using LC–MS. Characterization of the resulting conjugates was performed using SEC-MALS-QELS.
Utilizing Multidimensional LC–MS for Hydroxyl Radical Footprinting Analysis
November 01, 2019
Articles
37
11
The potential of multidimensional online peptide mapping analysis as a strategy for improving a postlabeling workflow for protein–protein interactions is demonstrated using both hydroxy radical footprinting–mass spectrometry (HRF–MS) and LC–MS/MS.
Development of a Size-Exclusion Chromatography Method to Characterize a Multimeric PEG–Protein Conjugate
November 01, 2019
Articles
37
11
A simple and robust size-exclusion chromatography (SEC) method has been developed for characterizing a multimeric PEG–protein conjugate. A wide range of size variants of the conjugate, ranging from 50 kDa to >1000 kDa, can be resolved and quantitated.