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Vaccines from plysaccharide materials

Despite the economic downturn, liquid chromatography (LC) will see significant growth in the coming years thanks to the technique's applicability in food safety testing, according to a market report by publisher Kalorama Information.

Waters has announced that the National Cancer Institute's Centre for Cancer Research in Bethesda, Maryland, USA, has purchased one of the company's ACQUITY UPLC/Synapt High Definition Mass Spectrometers (HDMS) and an ACQUITY UPLC/Xevo TQ MS system for the centre's metabolomics research programs.

The field of chromatographic analysis has evolved towards high throughput screening (HTS) in recent years. This trend has favoured the development of techniques and materials that allow shorter analysis times with high efficiency.

Joining us for this discussion are Michael P. Balogh of Waters Corporation; Mark Garner of Applied Biosystems; Michael McGinley of Phenomenex; and Jonathan McNally and Lester Taylor of Thermo Fisher Scientific.

The following question and answer are taken from LCGC Europe's "CE Currents" column (1). Does capillary electrophoresis (CE) sample solvent viscosity have an effect?

Varian, Inc. has published an application note entitled Separation of Trichlorobenzene from Sardines using EcoSpheres. The analysis of pesticides and other contaminants in environmental samples is an important part of monitoring the condition of the environment.

Use of Strata-X-C SPE cartridges allows for an aggressive organic wash of biological samples while maintaining high recoveries of amphetamines and their metabolites. In combination with LC/MS/MS, limits of quantitation (LOQ) of 0.54 ng/mL are achieved for MAMPH, HMMA, and MDMA and LOQ of 1.0 ng/mL are achieved for AMPH and MDA on Gemini C18 HPLC columns. Accuracy and reproducibility are not compromised with this simple and effective approach.

PSS and Tosoh Bioscience are taking EcoSEC - the semi-micro GPC/SEC system for high-throughput polymer analysis - on tour. The tour began at the end of March in Berlin and will visit 14 towns in Germany, Switzerland and the Netherlands before ending in the middle of June.

Hydrophilic interaction liquid chromatography (HILIC) is the preferred method for the separation of polar compounds. Tosoh Bioscience introduces a new, amino-type HILIC column with high stability.

The use of HPLC for determining amphetamines has not received much attention because of the poor response of UV spectrometric detectors to these compounds when present at low concentration levels. However, HPLC coupled with mass spectrometry can provide the sensitivity required for the analysis of analytes at low concentrations in biological samples. In comparison to GC–MS and LC-UV analyses, LC–MS–MS offers superior sensitivity, selectivity and robustness for a wide range of analytes without any need for derivatization. LC–MS–MS technology also makes possible the development of highly selective, sensitive and precise methods for the simultaneous analysis of amphetamines and their metabolic derivatives. In combination with LC–MS–MS, limits of quantitation (LOQ) of 0.54 ng/mL are achieved for MAMPH, HMMA, and MDMA and LOQ of 1.0 ng/mL are achieved for AMPH and MDA on Gemini C18 HPLC columns.

Varian, Inc. has published an application note entitled Separation of Trichlorobenzene from Sardines using EcoSpheres. This note illustrates how EcoSpheres can be used to assess contaminant levels in environmental samples.

HPTLC Determination of Polycyclic Aromatic Hydrocarbons in Mussels After a Crude Oil Spill in Chile Victor H. Campos, Katherine MuÖoz, Elizabeth Jara, Patricia Gonzalez, Mario Aranda and Mario Vega. Characterizing the Secreted Proteome of Mycobacterium Tuberculosis by LC–MS–MS Matthew M. Champion and Patricia A. DiGiuseppe Champion Meeting Review Pittcon 2009 This year's president, Ed Ladner, reflects on the success of Pittcon 2009 and demonstrates how the struggling global economy didn't take the shine away from its 60th celebration. News Protecting the Florida Everglades, securing nitrogen supplies, developments in ice chromatography and a discovery involving cheese are in the news this month. Incognito Pittcon Pioneers... After returning from Pittcon this year a little deflated by the lack of inspiration on offer, Incognito calls for a return to the inventive spirit and urges all entrepreneurs to demonstrate their creativity. Market Trends and Analysis Market Profile: Automated Peptide/Protein Sequencers Glenn Cudiamet Tips and Tricks: GPC/SEC How to choose a Static Light-Scattering Technique for the Molar Mass Determination Daniela Held and Peter Kilz Q&A It's a Small World The Column spoke to Job Elders of C2V about developments in micro gas chromatography.

Rapid Analysis of Limonin Citrus Extract Using U-HPLC-Hitachi Application Note

Calendar

In this article, the authors discuss the need for protection against chemical attacks and the role of passive imaging spectroradiometers in the detection of remote chemical agents.

Application of Novel Ethylene Bridged Hybrid Particles for Hydrophilic Interaction Chromatography- Waters Application Note

The use of mass spectrometry to study biofuels is examined and the advantages and disadvantages are explored.

Short Courses

This article discusses instruments that can be used in the field to rapidly and accurately identify various explosives and their precursors.

Application of Novel Ethylene Bridged Hybrid Particles for Hydrophilic Interaction Chromatography- Waters Application Note

Analysis of Products Containing the Sweetener Stevia-ESA Application Note

Researchers from Universitat Rovira i Virgili (Tarragona, Spain) and the University of Strathclyde (Glasgow, UK) synthesized two molecularly imprinted polymers (MIPs) by precipitation polymerization using the antiepileptic drug carbamazepine as the template molecule and two types of crosslinking agents.

Addressing the Acetonitrile Shortage-Waters Application Note

A Robust Validation Method for Guanidine-Microsolv Application Note

Fentanyl Quantitation Using Online High-throughput System-Thermo Application Note

Optimizing Sensitivity and Selectivity for the Analysis-Phenomenex Application Note

Scientists from the New Jersey Institute of Technology (Newark, New Jersey) used micro solid-phase extraction (SPE) devices consisting of single- and multiwalled carbon nanotubes packed in the needle of a syringe for sampling, analyte enrichment, and sample introduction.