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There is more to using and interpreting certificates for chromatography standards than meets the eye

Accurate, sensitive, and comprehensive characterization of monoclonal antibodies is an absolute necessity to the pharmaceutical and diagnostic industries to meet regulatory requirements and ensure the efficacy and safety of the final product. Microfluidic chip-based high performance liquid chromatography technology interfaced with the mass accuracy of quadrupole time-of-flight mass spectrometry provides the ability to rapidly and efficiently assess the quality of intact monoclonal antibodies, confirm their amino acid sequence, and determine their glycosylation state, while consuming very small amounts of these precious products.

The analysis of amines by gas chromatograph ;mass spectrometry (GC–MS) using electron ionization (EI) has always been a challenge

Two-dimensional gas chromatography–time-of-flight mass spectrometry (GCÃ-GC–TOF-MS) analysis has emerged as one of the technologies of choice for the analysis of small metabolite profiles. The results of these analyses produce substantial quantities of data that can be extremely time-consuming and labor-intensive for the analyst to interpret. New software provides a tool for the scientist to use as a data-mining strategy to find significant results from large, complex data sets. This proof of concept research was conducted using comprehensive GCÃ-GC–TOF-MS to elucidate the small-molecule metabolite profiles of diabetic and nondiabetic urine in search of key differences between disease-state and nondisease-state individuals.

Which is more important: signal or noise?

The International Symposium on High Performance Liquid Phase Separations and Related Techniques (the HPLC Symposium) is one of the leading international conferences in separation science. Since the first symposium in 1973, held in Interlaken, Switzerland, the conference has focused on bringing the latest in separation science theory, technology and applications to an international audience.

All of the most important sessions, awards and events from Pittcon 2010 covered here in this convenient and easy-to-read daily e-newsletter.

In preclinical development, the absolute quantification of peptides in biological matrices becomes a challenge due to the limited availability of stable label internal standards and affinity-based cleanup. This puts a renewed emphasis on matrix effects, especially for the bioanalysis of hydrophobic peptides. While the impact of matrix effects has been studied for extensively singly charged small molecules, their effect on multiply charged compounds has yet to be characterized fully. This article discusses initial results from matrix effect experiments in relation to the bioanalysis of hydrophobic peptides and techniques used to minimize matrix effects.

Hydrogen carrier gas can be an effective replacement for helium in many gas chromatography (GC) and GC–mass spectrometry (MS) applications.

Interchim has announced the acquisition of the flash chromatography branch of Armen Instrument, a French manufacturer of scientific instruments.

This month in "GC Connections," John Hinshaw discusses issues that can arise when deciding to upgrade or replace gas chromatography technologies.

What can be expected when the column diameter is changed?

The method developed runs on a fully automated platform integrating solid-phase extraction (SPE) and LC (Symbiosis Pico) without any manual pretreatment step(s). Automatic pretreatment results in high throughput, low CVs and high precision. Sample preparation has been simplified to just one operator step before the system takes over the automated analysis of the samples.

The regular maintenance of gas chromatography components, including symptons, causes and remedies.

Click the title above to open the LCGC North America March 2010 regular issue, Vol 28 No 3, in an interactive PDF format.

Tuesday?s morning session, ?Best Practice of Stability-Indicating HPLC Method Development,? was arranged by LCGC contributor Michael W. Dong of Genentech. Dong will begin the session with his opening remarks on the topic promptly at 8:00 a.m.

Buyer's Guide

GPC/SEC, often coupled with RI, Viscometry and/or LS detection, is the method of choice to characterize polymers and proteins.

This program will discuss the nature of GPC/SEC columns, describe how to read a chromatogram and help to identify column-caused artifacts and problems.