HILIC

Melamine is an organic base and a trimer of cyanamide, with a 1,3,5- triazine skeleton.

The analysis of anions and cations is critical during drug development and related QC. Measure both the API and the counterion in a single run.

The analysis of polar compounds in support of clinical and preclinical pharmacokinetic studies requires an analytical methodology capable of achieving ultra-low detection and quantification limits. The high sensitivity afforded by coupling HPLC with tandem mass spectrometry (MS-MS) has made it the technique of choice in this environment, but it is subject to the following limitations when reversed phase liquid chromatography (RPLC) is used:

The differences between retention of polar-ionic compounds on silica hydride-based and hydrophilic interaction liquid chromatography (HILIC) stationary phases are discussed. The retention capabilities of a number of silica hydride columns are reviewed, including their ability to operate in the reversed-phase mode. A means of differentiating between the two types of materials based upon their retenion modes is suggested.

Phenomenex

The terms hydrophilic interaction liquid chromatography (HILIC) and aqueous normal phase (ANP) chromatography very often are used interchangeably.

The authors work to develop a universal high performance liquid chromatography method that is capable of simultaneously retaining and separating both cations and anions within a single chromatographic analysis for the purpose of quantification in pharmaceutical products.