March 29th 2024
The 26th Norwegian Symposium on Chromatography was held 21–23 January 2024. The symposium has strong traditions in the Norwegian separation science community, serving as a forum for excellent scientific talks, networking, and social events.
Highly Efficient Separation of Bath Salts Using Hydrophilic Interaction Chromatography (HILIC)
September 1st 2011According to law enforcement and health officials, "bath salts" are powerful synthetic stimulants, designed to be comparable in effect to cocaine or methamphetamine, carrying similar risks. Unlike cocaine or methamphetamine, these stimulants are currently legal in many parts of the United States and available at a low cost in many convenience stores and head shops.
An Empirical Evaluation of HILIC and Monolithic Columns for SFC Applications
February 1st 2010Separation of polar compounds by conventional reverse phase chromatography can be challenging due to their poor retention. Other HPLC approaches include normal phase chromatography (NPC) and its variation, hydrophilic interaction chromatography (HILIC).
HILIC with Alcohol as the Organic Modifier
April 1st 2009Researchers from Amgen (Thousand Oaks, California) examined the separation mechanism in the analysis of polar and basic hydrazines using hydrophilic interaction liquid chromatography (HILIC) with alcohol rather than acetonitrile as a weak eluent.
Increasing LC–MS-MS Sensitivity with Luna® HILIC
June 1st 2008The analysis of polar compounds in support of clinical and preclinical pharmacokinetic studies requires an analytical methodology capable of achieving ultra-low detection and quantification limits. The high sensitivity afforded by coupling HPLC with tandem mass spectrometry (MS-MS) has made it the technique of choice in this environment, but it is subject to the following limitations when reversed phase liquid chromatography (RPLC) is used:
Silica Hydride-Based Stationary Phases: Not Just Another HILIC Column
May 30th 2008The differences between retention of polar-ionic compounds on silica hydride-based and hydrophilic interaction liquid chromatography (HILIC) stationary phases are discussed. The retention capabilities of a number of silica hydride columns are reviewed, including their ability to operate in the reversed-phase mode. A means of differentiating between the two types of materials based upon their retenion modes is suggested.