March 29th 2024
The 26th Norwegian Symposium on Chromatography was held 21–23 January 2024. The symposium has strong traditions in the Norwegian separation science community, serving as a forum for excellent scientific talks, networking, and social events.
Development of an Ultrasensitive LC–MS-MS Method for Determination of 5-Fluorouracil in Mouse Plasma
5-Fluorouracil (5-FU) is a low-molecular-weight anticancer drug in clinical use for several solid tumors in humans. Currently, the most widely used methodology for 5-FU quantitation is liquid chromatography–tandem mass spectrometry (LC–MS-MS) with either liquid–liquid extraction (LLE), protein precipitation, or a combination of both as sample cleanup procedures.
HILIC: A Powerful Separation Mode for a Broad Range of Applications
September 19th 2013The rising interest in HILIC can be demonstrated by the growing number of publications and applications over the last 10 years. Applications range from food and nutrition, to cosmetics, pharmaceuticals, and bioanalysis.
Advances in Hydrophilic Interaction Liquid Chromatography for Pharmaceutical Analysis
May 2nd 2013Hydrophilic interaction liquid chromatography (HILIC) has recently become more important, particularly for the analysis of polar drugs, metabolites and biologically relevant compounds in glycomics, proteomics, metabolomics and clinical analysis. HILIC makes it possible to increase the retention of polar compounds, achieve orthogonal selectivity and increase mass spectrometry (MS) sensitivity, compared with reversed-phase liquid chromatography. This article discusses the advantages and limitations of HILIC in a variety of practical applications in the pharmaceutical industry.
Advantages of HILIC Mobile Phases for LC–ESI–MS–MS Analysis of Neurotransmitters
March 1st 2013The aim of this work was to find the optimal conditions to achieve sufficient limits of detection (LOD) that would permit the detection of neurotransmitters by LC–MS–MS in biological samples. An optimized HILIC–ESI–MS–MS system for the analysis of the 12 selected compounds was proposed.
The LCGC Blog: Chromatography Technical Tips - Hydrophilic Interaction Liquid Chromatography (HILIC)
February 11th 2013The Technical Department at Crawford Scientific are regularly asked for analytical conditions or applications in the hydrophilic interaction liquid chromatography (HILIC) mode. Here?s a short primer on what you need to understand about this separation mode from a practical perspective, using some of the questions that we regularly get asked.
Determination of Histamine in Human Serum/Plasma by SPE and HILIC LC-MS-MS
December 5th 2012Reversed-phase HPLC analysis of histamine has been a challenge in the past due to the high polarity and poor retention. HILIC (hydrophilic interaction chromatography) offers a potential solution because of high polar analyte retention without the need of strong ion-pair reagents, which are not compatible with mass spectrometry (MS) detection and can cause signal suppression.
HILIC: An Alternative Approach for Separating Highly Polar Analytes
November 15th 2012Hydrophilic interaction chromatography (HILIC) is an alternative separation technique for highly polar analytes that are weakly retained in reversed-phase high performance liquid chromatography (HPLC). Participants in this HILIC Technology Forum are William Long of Agilent Technologies, Phil Koerner and Carl Sanchez of Phenomenex, and Nicole M. Cotta of Waters Corp.
Separation of 20 Amino Acids by Polymer-Based Amino HILIC Column
September 1st 2012Determination and quantification of amino acids are important in many fields. For the HPLC analysis of amino acids, cation exchange mode method has been used with post-column derivatization. Recently, use of reversed-phase mode method is also increasing. However, because of amino acids' hydrophilic nature, they are usually not well retained and have less selectivity by reversed-phase mode without pre-column derivatization steps. In contrast, HILIC mode is an ideal separation mode for hydrophilic compound analysis.
The Hyphenation of HILIC, Reversed-Phase HPLC, and Atmospheric-Pressure-Ionization MS
May 1st 2012The coupling of HILIC with reversed-phase HPLC and API-MS will become routine in the future, particularly in fields like metabolomics and environmental analysis that regularly involve nontargeted screening of large numbers of compounds in complex mixtures.