HILIC

Due to the highly polar nature of saccharides, the analysis of sugars is typically achieved using hydrophilic interaction chromatography (HILIC).

HILIC was introduced more than 50 years ago but has attracted more attention recently. Giorgia Greco from the Technische Universität Munchen in Germany discusses the different options available for combining HILIC to reversed-phase LC, and how HILIC can be hyphenated with atmospheric pressure chemical ionization MS.

Due to the highly polar nature of saccharides, the analysis of sugars is typically achieved using Hydrophilic Interaction Chromatography (HILIC). Using a high polarity packing material, separation is based on the hydrophilic interactions of the sugars on the stationary phase surface typically using an eluent with a majority of polar organic solvent.

Understanding the interactions that stationary-phase chemistries provide will help you choose the most appropriate blend of interactions to address a given separation challenge.

The retention and selectivity of stationary phases in HILIC is discussed.

Davy Guillarme, a senior lecturer at the School of Pharmaceutical Sciences at the University of Geneva, University of Lausanne, Switzerland, answers common user questions about HILIC.

Due to the highly polar nature of saccharides, the analysis of sugars is typically achieved using hydrophilic interaction chromatography (HILIC).

When you begin to now combine advances in superficially porous media to achieve high efficiency with novel and predictable new stationary phases to achieve high selectivity, then it sounds like the evolution of HPLC and UHPLC phases over the past decade or more has brought some exciting new possibilities.

Hydrophilic interaction chromatography (HILIC) provides an alternative approach for the separation of highly polar substances.

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This article, transcribed from an LCGC web seminar presented on January 29, 2013, by Davy Guillarme of the University of Geneva and the University of Lyon, explains how HILIC works and provides keys to using the technique effectively.

A Critical Evaluation of Hydrophilic-Interaction Chromatography (HILIC)

The modern usage and specific applications of HILIC for two other major classes of analytes - glycopeptides and glycoproteins - are discussed.

Answers to common questions about HILIC, and an overview of its application to biopharmaceutical analysis.

5-Fluorouracil (5-FU) is a low-molecular-weight anticancer drug in clinical use for several solid tumors in humans. Currently, the most widely used methodology for 5-FU quantitation is liquid chromatography–tandem mass spectrometry (LC–MS-MS) with either liquid–liquid extraction (LLE), protein precipitation, or a combination of both as sample cleanup procedures.

The rising interest in HILIC can be demonstrated by the growing number of publications and applications over the last 10 years. Applications range from food and nutrition, to cosmetics, pharmaceuticals, and bioanalysis.

EMD Millipore

Agilent Technologies Inc.

Davy Guillarme discussed the possibilities and limitations of HILIC in a recent web seminar. Here, he answers common questions about the method.

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Agilent Technologies, Inc

Davy Guillarme discussed the possibilities and limitations of HILIC in a recent web seminar. Here, he answers common questions about the method.

Click here to view the complete E-Separation Solutions newsletter from May 23, 2013.

Hydrophilic interaction liquid chromatography (HILIC) has recently become more important, particularly for the analysis of polar drugs, metabolites and biologically relevant compounds in glycomics, proteomics, metabolomics and clinical analysis. HILIC makes it possible to increase the retention of polar compounds, achieve orthogonal selectivity and increase mass spectrometry (MS) sensitivity, compared with reversed-phase liquid chromatography. This article discusses the advantages and limitations of HILIC in a variety of practical applications in the pharmaceutical industry.

The aim of this work was to find the optimal conditions to achieve sufficient limits of detection (LOD) that would permit the detection of neurotransmitters by LC–MS–MS in biological samples. An optimized HILIC–ESI–MS–MS system for the analysis of the 12 selected compounds was proposed.

The Technical Department at Crawford Scientific are regularly asked for analytical conditions or applications in the hydrophilic interaction liquid chromatography (HILIC) mode. Here?s a short primer on what you need to understand about this separation mode from a practical perspective, using some of the questions that we regularly get asked.

Reversed-phase HPLC analysis of histamine has been a challenge in the past due to the high polarity and poor retention. HILIC (hydrophilic interaction chromatography) offers a potential solution because of high polar analyte retention without the need of strong ion-pair reagents, which are not compatible with mass spectrometry (MS) detection and can cause signal suppression.

Agilent Technologies