September 20th 2024
Here is some of the most popular content posted on LCGC International this week.
Recent Developments in HPLC & UHPLC
June 5th 2024In the world of liquid chromatography, innovative strides in column technology continue to take place. We are also reminded that there is always more to learn about “well-known” methodologies, and our craft is continuously influenced by important social concerns.
The 26th Norwegian Symposium on Chromatography
March 29th 2024The 26th Norwegian Symposium on Chromatography was held 21–23 January 2024. The symposium has strong traditions in the Norwegian separation science community, serving as a forum for excellent scientific talks, networking, and social events.
New 2D LC–MS Analytical Method Unveils the Secrets of Ancient Chinese Herbal Formula HGWD
October 10th 2023A comprehensive analytical approach utilizing online two-dimensional liquid chromatography coupled with hybrid linear ion trap orbital trap mass spectrometry, has enabled the in-depth analysis of Huangqi Guizhi Wuwu decoction, identifying 170 chemical constituents and offering insights into its complex chemical composition and potential therapeutic properties.
Polysorbate Analysis in Biopharmaceutical Applications—A Snapshot of the Analytical Toolbox
An overview of different approaches for the qualitative and quantitative analysis of polysorbates.
Strategies for Overcoming Sample Introduction Challenges in Hydrophilic-Interaction Chromatography
July 13th 2023A team of researchers has investigated strategies to overcome sample introduction challenges in hydrophilic-interaction chromatography (HILIC). By employing techniques such as pre-column dilution and selecting alternative organic solvents, they successfully addressed the issues related to injection solvent strength, resulting in improved peak symmetry and separation efficiency in HILIC analyses.
Successful Analysis of Protein Biopharmaceuticals Using Ultra-Short Columns
June 20th 2023Davy Guillarme of the University of Geneva said in a talk at HPLC 2023 that short columns have shown promise, but ultrahigh-performance liquid chromatography (UHPLC) instrumentation is not yet optimized to realize their benefits.
Adjustable Polyacrylamide Stationary Phases Enhance Separation Performance in Liquid Chromatography
June 12th 2023Adjustable polyacrylamide stationary phases developed by researchers enhance separation performance in liquid chromatography, according to a recent publication. The study demonstrates the synthesis of vinyl modified silica and the successful preparation of polyacrylamide modified silica (PAM-SIL) stationary phases, allowing for fine control of separation performance.
An Interview with AI About Its Potential Role in Separation Science
May 17th 2023We interviewed an AI program (ChatGPT) for LCGC North America asking questions about AI and its role in various applications for separation science to include data analysis, and high performance liquid chromatography (HPLC), hydrophilic-interaction chromatography (HILIC), reversed-phase liquid chromatography (RPLC), liquid chromatography–mass spectrometry (LC–MS), gas chromatography–mass spectrometry (GC–MS), high resolution mass spectrometry (HRIM–MS), high resolution tandem mass spectrometry (HRMS/MS), and related topics.
An LC–MS/MS Method for the Evaluation of Intestinal Permeability in Blood Plasma
May 1st 2023A rapid and robust LC–MS/MS method for determining lactulose and rhamnose concentrations in blood plasma was used to determine intestinal permeability from blood plasma, which can help diagnose gastrointestinal diseases such as Crohn’s disease.
New Nickel Oxide Stationary Phase for Improved HILIC Separation
April 27th 2023A new study has reported the preparation, characterization, and chromatographic evaluation of a nickel oxide deposited silica stationary phase in HILIC, which exhibited multiple retention mechanisms and enhanced chromatographic selectivity for analytes.