Accurate, High Sensitivity Analysis of Impurities

September 1, 2010
Darwin Asa

ESA Biosciences, Inc.

LCGC Asia Pacific

LCGC Asia Pacific, LCGC Asia Pacific-09-01-2010, Volume 13, Issue 3
Pages: 38–39

Bruker Daltonics Application Note

Modern analytical applications often demand definitive tandem MS results on ever more complex samples using fast separation techniques. maXis is the only mass spectrometer able to deliver the maximum MS performance specification at the very highest speeds delivered by modern ultra performance liquid chromatography (UPLC) and capillary electrophoresis (CE). The maXis is specially designed to deliver excellent results in many applications including:

• Small molecule identification.

• Impurity and degradent identification.

• In vitro and in vivo drug metabolite identification.

• Intact protein analysis.

• Quantitative proteomics and protein identification.

Figure 1: Dilution series of okadaic acid from 0.1 to 100 ppb.

Redefining High Performance Mass Spectrometry

With resolution in excess of 40,000 and MS and MS–MS mass accuracy typically between 600–800 ppb at speeds of up to 20 full spectra per second simultaneously, no other mass spectrometer is better equipped to deliver definitive data on complex samples in proteomics, metabolomics and small molecule identification challenges.

Figure 2: Mass Accuracy determination for a variety of steroid molecules at 100 ppb.

Typical Results from the maXis

• 20 Hz speed of acquisition at high resolution for high-speed chromatography.

• 40 k+ resolution in both MS and MS–MS mode.

• Wide dynamic range of 5 orders of magnitude for trace detection in complex mixtures.

• Sub-ppm mass accuracy in both MS and MS–MS mode for high confidence i.d.s.

Figure 3: Extracted Ion Chromatograms of 0.1 ppb of various steroids.

Sensitivity and Dynamic Range Determination

In this study, the maXis was challenged to measure low level impurities. The maXis was easily able to detect compounds at 0.1 ppb and exhibited at least a four order of magnitude dynamic range for quantification. In addition, when challenged with even co-eluting compounds of several orders of magnitude difference in concentration, the maXis was readily able to identify and measure both compounds despite the difference in concentration. The maXis would seem to meet many criteria for use as an instrument to measure and detect low level impurities in terms of sensitivity, dynamic range and mass accuracy.

Figure 4: Analysis of a co-eluting impurity. In this example Dinorvenlafaxine and Norvenlafaxine co-elute with a difference of 250 × in concentration, but the maXis detects and identifies both despite the difference in concentration.

Bruker Daltonics Inc.

Billerica, Massachusetts 01824, USA

tel. +1 978 663 3660 fax +1 978 667 5993