Developing Better GC Methods — A Blueprint

December 1, 2014
Tony Taylor
Tony Taylor

Tony Taylor is Group Technical Director of Crawford Scientific Group and CHROMacademy. His background is in pharmaceutical R&D and polymer chemistry, but he has spent the past 20 years in training and consulting, working with Crawford Scientific Group clients to ensure they attain the very best analytical science possible. He has trained and consulted with thousands of analytical chemists globally and is passionate about professional development in separation science, developing CHROMacademy as a means to provide high-quality online education to analytical chemists. His current research interests include HPLC column selectivity codification, advanced automated sample preparation, and LC–MS and GC–MS for materials characterization, especially in the field of extractables and leachables analysis.

LCGC Europe

LCGC Europe, LCGC Europe-12-01-2014, Volume 27, Issue 12

Pages 678

An excerpt from LCGC's e-learning tutorial on developing better GC methods at

To obtain sensitive, robust, and reproducible gas chromatography (GC) methods, each stage of the chromatographic process needs to be carefully considered and optimized. It is also important to record and report as much detail within the method specification so that the method can be reproduced between operators, instruments, and laboratories. Table 1 represents a "blueprint" method specification with all of the information that is necessary to faithfully specify and reproduce a split–splitless GC method. Table 2 provides a blueprint starting point for the method development of a sample with unknown composition, but known to contain "trace" target analytes. Even if you are not developing methods - check the blueprint specifications against your GC methods. Do your methods contain all of the necessary details?

Table 1: Requirements for a properly specified splitless gas chromatography method with flame ionization detection (FID).

Table 2: Blueprint method specification for initial method development of a trace analysis using FID.