Guide Decisions through Drug Development: Localize Critical PTMs at the Sub-Unit Level



Webinar Date/Time: Wed, Jul 24, 2024 12:00 PM EDT

Discover a powerful, high-speed workflow for developability assessment during clone selection, manufacturing scale-up and QC of protein therapeutics. Gain information for confident identification and localization of multiple critical PTMs utilizing the Intabio ZT system for in depth subunit analysis.

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Event Overview:

With increasing complexity of novel biopharmaceuticals comes new challenges in developing stable cell lines to produce these treatements. Strategies to gain more information on critical post translational modifications in early development can significantly accelerate drug development timelines. Critical information on drug molecules at the intact level can reduce risk and streamline both downstream and upstream processes. This can be achieved by reducing the time required to gain this information and enabling the use of mass spectrometry assays at critical stages in early development.

This webinar will cover the use of an integrated icIEF-UV/MS workflow on the Intabio ZT system to identify multiple critical post translational modifications (PTMs) at the subunit level. Information gained from this 2-dimensional analysis provides glycan localization, deamidation, C terminal processing, N terminal cyclization, N linked glycosylation with terminal alpha Galactose and neuraminic acid, expanding on the core charge variant characterization applications of the Intabio ZT system.

Key Learning Objectives:

  • Explore the benefits of gaining PTM information at the subunit level for recombinant proteins
  • Discover a novel strategy for subunit analysis throughout development
  • Localize critical PTMs at the sub-unit level to assess potential impact on drug efficacy

Who Should Attend:

  • Drug development scientists
  • Lab directors
  • CDMO, CMO, CRO scientists
  • Senior scientists


Scott Mack
Principal Scientist

Scott Mack is a Principal Scientist and co-inventor of the Microchip Electrophoresis technology employed in the Intabio ZT system from SCIEX. Prior to joining SCIEX, Scott led icIEF assay development and system integration efforts on the Maurice System. In addition, he developed the advanced cIEF methodology for the PA 800 Plus system and pioneered enhanced cIEF-MS analysis with David Chen’s Lab at the University of British Columbia

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