The Column
A systematic way to develop HPLC methods consists of building up and running a useful set of experiments (DOE, design of experiments) to evaluate the influence of several factors or method conditions on the selectivity of a chromatogram. The crucial part here is the peak tracking between the chromatograms.
A systematic way to develop HPLC methods consists of building up and running a useful set of experiments (DOE, design of experiments) to evaluate the influence of several factors or method conditions on the selectivity of a chromatogram. The crucial part here is the peak tracking between the chromatograms. It has been shown that under most conditions peak areas can be used to identify peaks of the same compound in different chromatograms. However, this technique also has some limitations that will be discussed in this article.
High-Throughput 4D TIMS Method Accelerates Lipidomics Analysis
April 25th 2024Ultrahigh-pressure liquid chromatography coupled to high-resolution mass spectrometry (UHPLC-HRMS) had been previously proposed for untargeted lipidomics analysis, but this updated approach was reported by the authors to reduce run time to 4 min.
Traditional Chinese Medicine’s Effects on Liver Cancer Measured Using New UHPLC–QTOF-MS System
April 22nd 2024Scientists from Anhui University of Chinese Medicine in Hefei, China recently investigated the mechanisms behind what makes huachansu tablets, a type of traditional Chinese medicine (TCM), effective against liver cancer.