|Articles|November 1, 2001

LCGC North America

  • LCGC North America-11-01-2001
  • Volume 19
  • Issue 11

What Drives a Separation — the Stationary Phase or the Mobile Phase?

The author investigates solute partitioning between the mobile and stationary phases to determine the driving force of reversed-phase liquid chromatography separations.

Articles in this issue

almost 25 years ago

Striving to Validate Nutraceuticals

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Marketplace

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Retaining Polar Compounds

almost 25 years ago

Handling Fast Peaks

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Chromatography Detects Toxins in Tea Samples
High-performance liquid chromatography-tandem mass spectroscopy (HPLC–MS/MS) chromatography reveals pyrrolizidine alkaloids (PA)/pyrrolizidine alkaloid N-oxides (PANO) toxin risks in 63 tea samples.
Figure 2. Split injection used to measure triplicate test of MTBE, hexane, o-xylene, and 1-methylnaphthalene comparing different solvents (methanol, methylene chloride) and column insertion distances starting with 0.5 mm, 5.0 mm, 10.0 mm, 15 mm, and 20.0 mm. Methanol had the best performance at the 5.0 mm insertion distance and methylene chloride looked slightly better at the 0.5 mm. We would still recommend not going below the 5.0 mm manufacturer recommended insertion distance.
Revisiting insertion distance for methanol and methylene chloride in GC analysis and confirming that insertion depth strongly affects response and reproducibility.